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Validation Data Gallery

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  • Western Blot (WB) analysis of SH-SY5Y cells using FUBP1 Polyclonal antibody (24864-1-AP)

    WB analysis of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of K-562 cells using FUBP1 Polyclonal antibody (24864-1-AP)

    WB analysis of K-562 using 24864-1-AP (same clone as 24864-1-PBS)

    K-562 cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse brain tissue using FUBP1 Polyclonal antibody (24864-1-AP)

    WB analysis of mouse brain using 24864-1-AP (same clone as 24864-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FUBP1 Polyclonal antibody (24864-1-AP)

    WB analysis of Jurkat using 24864-1-AP (same clone as 24864-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 24864-1-AP (FUBP1 Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of SH-SY5Y cells using FUBP1 Polyclonal antibody (24864-1-AP)

    IP experiment of SH-SY5Y using 24864-1-AP (same clone as 24864-1-PBS)

    IP result of anti-FUBP1 (IP:24864-1-AP, 4ug; Detection:24864-1-AP 1:3000) with SH-SY5Y cells lysate 1480 ug. This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FUBP1 Polyclonal antibody (24864-1-AP)

    IF Staining of HeLa using 24864-1-AP (same clone as 24864-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FUBP1 antibody (24864-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red). This data was developed using the same antibody clone with 24864-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FUBP1 Polyclonal antibody, PBS Only

FUBP1 Polyclonal Antibody for WB, IF/ICC, IP, Indirect ELISA
Cat No. 24864-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, Indirect ELISA

Synonyms

Far upstream element-binding protein 1, FUSE-binding protein 1

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

24864-1-PBS targets FUBP1 in WB, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag13149

Product name: Recombinant human FUBP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 303-653 aa of BC017247

Sequence: DAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQCRFDPASIELAL

相同性解析による交差性が予測される生物種
Full Name far upstream element (FUSE) binding protein 1
Calculated molecular weight 653 aa, 69 kDa
Observed molecular weight 69 kDa
GenBank accession numberBC017247
Gene Symbol FUBP1
Gene ID (NCBI) 8880
RRIDAB_2879762
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ96AE4
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FUBP1 also termed as FBP and FUSE-binding protein 1 is 644 amino-acid protein, which localizes in the nucleus. FUBP1 binds to a single-stranded far-upstream element (FUSE) upstream of the MYC promoter and regulates the MYC expression, indicating that FBP1 functions as a growth-dependent regulator of c-Myc expression. FUBP1 binds to FUSE, and PUF60, and forms a stable tripartite complex, which represses activated but not basal c-myc transcription after transcription initiation by delaying promoter escape. FUBP1 may be acting both as activator and repressor of transcription.

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