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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    WB analysis using 28218-1-AP (same clone as 28218-1-PBS)

    Human placenta tissues were subjected to SDS PAGE followed by western blot with 28218-1-AP (FSTL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    WB analysis of HeLa using 28218-1-AP (same clone as 28218-1-PBS)

    WB result of FSTL3 antibody (28218-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL3 transfected HeLa cells. This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of human urothelial carcinoma using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human urothelial carcinoma tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of human urothelial carcinoma using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of rat kidney using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of rat kidney using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of rat testis using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat testis tissue using FSTL3/FLRG Polyclonal antibody (28218-1-AP)

    IHC staining of rat testis using 28218-1-AP (same clone as 28218-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28218-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FSTL3/FLRG Polyclonal antibody, PBS Only

FSTL3/FLRG Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 28218-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

28218-1-PBS targets FSTL3/FLRG in WB, IHC, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag27626

Product name: Recombinant human FSTL3 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 94-263 aa of BC005839

Sequence: PCKDSCDGVECGPGKACRMLGGRPRCECAPDCSGLPARLQVCGSDGATYRDECELRAARCRGHPDLSVMYRGRCRKSCEHVVCPRPQSCVVDQTGSAHCVVCRAAPCPVPSSPGQELCGNNNVTYISSCHMRQATCFLGRSIGVRHAGSCAGTPEEPPGGESAEEEENFV

相同性解析による交差性が予測される生物種
Full Name follistatin-like 3 (secreted glycoprotein)
Calculated molecular weight 263 aa, 28 kDa
Observed molecular weight28-33 kDa
GenBank accession numberBC005839
Gene Symbol FSTL3
Gene ID (NCBI) 10272
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95633
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.

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