Validation Data Gallery
Tested Applications
| Positive WB detected in | human placenta tissue, HeLa cells |
| Positive IHC detected in | human urothelial carcinoma tissue, rat kidney tissue, rat testis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28218-1-AP targets FSTL3/FLRG in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag27626 Product name: Recombinant human FSTL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 94-263 aa of BC005839 Sequence: PCKDSCDGVECGPGKACRMLGGRPRCECAPDCSGLPARLQVCGSDGATYRDECELRAARCRGHPDLSVMYRGRCRKSCEHVVCPRPQSCVVDQTGSAHCVVCRAAPCPVPSSPGQELCGNNNVTYISSCHMRQATCFLGRSIGVRHAGSCAGTPEEPPGGESAEEEENFV 相同性解析による交差性が予測される生物種 |
| Full Name | follistatin-like 3 (secreted glycoprotein) |
| Calculated molecular weight | 263 aa, 28 kDa |
| Observed molecular weight | 28-33 kDa |
| GenBank accession number | BC005839 |
| Gene Symbol | FSTL3 |
| Gene ID (NCBI) | 10272 |
| RRID | AB_3086037 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95633 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FSTL3/FLRG antibody 28218-1-AP | Download protocol |
| WB protocol for FSTL3/FLRG antibody 28218-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |







