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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FPGS Polyclonal antibody (27616-1-AP)

    WB analysis using 27616-1-AP (same clone as 27616-1-PBS)

    HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of mouse kidney using 27616-1-AP (same clone as 27616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of human lung cancer using 27616-1-AP (same clone as 27616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of human lung cancer using 27616-1-AP (same clone as 27616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of mouse kidney using 27616-1-AP (same clone as 27616-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 27616-1-PBS in a different storage buffer formulation.

FPGS Polyclonal antibody, PBS Only

FPGS Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27616-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Tetrahydrofolate synthase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27616-1-PBS targets FPGS in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24775

Product name: Recombinant human FPGS protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 397-587 aa of BC064393

Sequence: QGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSVPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ

相同性解析による交差性が予測される生物種
Full Name folylpolyglutamate synthase
Calculated molecular weight 65 kDa
Observed molecular weight60-65 kDa
GenBank accession numberBC064393
Gene Symbol FPGS
Gene ID (NCBI) 2356
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05932
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

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