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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FPGS Polyclonal antibody (27616-1-AP)

    WB analysis using 27616-1-AP

    HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HeLa cells using FPGS Polyclonal antibody (27616-1-AP)

    WB analysis of HeLa using 27616-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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FPGS Polyclonal antibody

FPGS Polyclonal Antibody for WB, ELISA
Cat No. 27616-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inHeLa mitochondrial extract

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27616-1-AP targets FPGS in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FPGS fusion protein Ag24775 相同性解析による交差性が予測される生物種
Full Name folylpolyglutamate synthase
Calculated molecular weight 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC064393
Gene Symbol FPGS
Gene ID (NCBI) 2356
RRIDAB_3669615
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05932
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

Protocols

Product Specific Protocols
WB protocol for FPGS antibody 27616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols