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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FPGS Polyclonal antibody (27616-1-AP)

    WB analysis using 27616-1-AP

    HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of mouse kidney using 27616-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of human lung cancer using 27616-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of human lung cancer using 27616-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FPGS Polyclonal antibody (27616-1-AP)

    IHC staining of mouse kidney using 27616-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 27616-1-AP (FPGS antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FPGS Polyclonal antibody

FPGS Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27616-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Tetrahydrofolate synthase

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa mitochondrial extract
Positive IHC detected inmouse kidney tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27616-1-AP targets FPGS in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag24775

Product name: Recombinant human FPGS protein

Source: e coli.-derived, PET30a

Tag: 6*His

Domain: 397-587 aa of BC064393

Sequence: QGRERPSGGPEVRVLLFNATGDRDPAALLKLLQPCQFDYAVFCPNLTEVSSTGNADQQNFTVTLDQVLLRCLEHQQHWNHLDEEQASPDLWSVPSPEPGGSASLLLAPHPPHTCSASSLVFSCISHALQWISQGRDPIFQPPSPPKGLLTHPVAHSGASILREAAAIHVLVTGSLHLVGGVLKLLEPALSQ

相同性解析による交差性が予測される生物種
Full Name folylpolyglutamate synthase
Calculated molecular weight 65 kDa
Observed molecular weight65 kDa
GenBank accession numberBC064393
Gene Symbol FPGS
Gene ID (NCBI) 2356
RRIDAB_3669615
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ05932
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.

Protocols

Product Specific Protocols
WB protocol for FPGS antibody 27616-1-APDownload protocol
IHC protocol for FPGS antibody 27616-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols