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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO6 Recombinant antibody (83926-1-RR)

    WB analysis using 83926-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83926-1-RR (FOXO6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FOXO6 Recombinant antibody (83926-1-RR)

    IHC staining of rat brain using 83926-1-RR

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83926-1-RR (FOXO6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using FOXO6 Recombinant antibody (83926-1-RR)

    IF Staining of rat brain using 83926-1-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FOXO6 Recombinant antibody (83926-1-RR)

    IF Staining of A549 using 83926-1-RR

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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FOXO6 Recombinant antibody

FOXO6 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 83926-1-RR
Clone No.240823D10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

forkhead box protein O6, 240823D10

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHeLa cells, HepG2 cells
Positive IHC detected inrat brain tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inrat brain tissue
Positive IF/ICC detected inA549 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83926-1-RR targets FOXO6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide 相同性解析による交差性が予測される生物種
Full Name forkhead box protein O6
Calculated molecular weight 51 kDa
Observed molecular weight75 kDa
GenBank accession numberAI361654
Gene Symbol FOXO6
Gene ID (NCBI) 100132074
RRIDAB_3671506
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system.

Protocols

Product Specific Protocols
WB protocol for FOXO6 antibody 83926-1-RRDownload protocol
IHC protocol for FOXO6 antibody 83926-1-RRDownload protocol
IF protocol for FOXO6 antibody 83926-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols