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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FOXO6 Recombinant antibody (83926-1-RR)

    WB analysis using 83926-1-RR (same clone as 83926-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83926-1-RR (FOXO6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83926-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brain tissue using FOXO6 Recombinant antibody (83926-1-RR)

    IHC staining of rat brain using 83926-1-RR (same clone as 83926-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83926-1-RR (FOXO6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83926-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of rat brain tissue using FOXO6 Recombinant antibody (83926-1-RR)

    IF Staining of rat brain using 83926-1-RR (same clone as 83926-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83926-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A549 cells using FOXO6 Recombinant antibody (83926-1-RR)

    IF Staining of A549 using 83926-1-RR (same clone as 83926-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83926-1-PBS in a different storage buffer formulation.

FOXO6 Recombinant monoclonal antibody, PBS Only

FOXO6 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA
Cat No. 83926-1-PBS
Clone No.240823D10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

forkhead box protein O6, 240823D10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83926-1-PBS targets FOXO6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

Peptide

相同性解析による交差性が予測される生物種
Full Name forkhead box protein O6
Calculated molecular weight 51 kDa
Observed molecular weight75 kDa
GenBank accession numberAI361654
Gene Symbol FOXO6
Gene ID (NCBI) 100132074
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDA8MYZ6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system.

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