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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated FNTB Monoclonal antibody

FNTB Monoclonal Antibody for IF, FC (Intra)

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse

Applications

IF, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

1F3B10

Cat no : CL594-66783

Synonyms

FNTB, FPTB, FTase beta

Validation Data Gallery

Applications:
  • IF Staining of A431 using CL594-66783

    IF Staining of A431 using CL594-66783

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

  • IF Staining of A431 using CL594-66783

    IF Staining of A431 using CL594-66783

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CoraLite®594 FNTB antibody (CL594-66783, Clone: 1F3B10 ) at dilution of 1:400.

  • FC experiment of A431 using CL594-66783

    FC experiment of A431 using CL594-66783

    1X10^6 A431 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human FNTB (CL594-66783, Clone:1F3B10) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

Tested Applications

Positive IF detected inA431 cells
Positive FC detected inA431 cells
For other applications, we recommend the unconjugated version of this antibody, 66783-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:200-1:800
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

CL594-66783 targets FNTB in IF, FC (Intra) applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen FNTB fusion protein Ag27796 相同性解析による交差性が予測される生物種
Full Name farnesyltransferase, CAAX box, beta
Calculated molecular weight 437 aa, 49 kDa
Observed molecular weight 44-45 kDa
GenBank accession numberBC020232
Gene symbol FNTB
Gene ID (NCBI) 2342
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

Protocols

Product Specific Protocols
IF protocol for CL594 FNTB antibody CL594-66783Download protocol
FC protocol for CL594 FNTB antibody CL594-66783Download protocol
Standard Protocols
Click here to view our Standard Protocols