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Validation Data Gallery

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  • Western Blot (WB) analysis of HEK-293 cells using FKBPL Polyclonal antibody (10060-1-AP)

    WB analysis of HEK-293 using 10060-1-AP (same clone as 10060-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human testis tissue using FKBPL Polyclonal antibody (10060-1-AP)

    WB analysis of human testis using 10060-1-AP (same clone as 10060-1-PBS)

    human testis tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FKBPL Polyclonal antibody (10060-1-AP)

    WB analysis of HeLa using 10060-1-AP (same clone as 10060-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of human brain tissue using FKBPL Polyclonal antibody (10060-1-AP)

    WB analysis of human brain using 10060-1-AP (same clone as 10060-1-PBS)

    human brain tissue were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of Jurkat cells using FKBPL Polyclonal antibody (10060-1-AP)

    WB analysis of Jurkat using 10060-1-AP (same clone as 10060-1-PBS)

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10060-1-AP (FKBPL antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of MCF-7 cells using FKBPL Polyclonal antibody (10060-1-AP)

    IP experiment of MCF-7 using 10060-1-AP (same clone as 10060-1-PBS)

    IP result of anti-FKBPL (IP:10060-1-AP, 3ug; Detection:10060-1-AP 1:800) with MCF-7 cells lysate 2500ug. This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBPL Polyclonal antibody (10060-1-AP)

    IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FKBPL Polyclonal antibody (10060-1-AP)

    IHC staining of human breast cancer using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBPL Polyclonal antibody (10060-1-AP)

    IHC staining of human testis using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human testis tissue using FKBPL Polyclonal antibody (10060-1-AP)

    IHC staining of human testis using 10060-1-AP (same clone as 10060-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human testis tissue slide using 10060-1-AP (FKBPL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FKBPL Polyclonal antibody (10060-1-AP)

    FC experiment of HeLa using 10060-1-AP (same clone as 10060-1-PBS)

    1x10^6 HeLa cells were intracellularly stained with 0.4 ug FKBPL Polyclonal antibody (10060-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 10060-1-PBS in a different storage buffer formulation.

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  • Featured Product
  • KD/KO Validated

FKBPL Polyclonal antibody, PBS Only

FKBPL Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA
Cat No. 10060-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), IP, ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10060-1-PBS targets FKBPL in WB, IHC, FC (Intra), IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FKBPL fusion protein Ag0112 相同性解析による交差性が予測される生物種
Full Name FK506 binding protein like
Calculated molecular weight 349 aa, 38 kDa
Observed molecular weight 42-48 kDa
GenBank accession numberBC004168
Gene Symbol FKBPL
Gene ID (NCBI) 63943
RRIDAB_2262677
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9UIM3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FKBPL, also named as DIR1, NG7 and WISp39, has similarity to the immunophilin protein family, which plays a role in immunoregulation and basic cellular processes involving protein folding and trafficking. FKBPL levels may be a prognostic indicator and determinant of response to endocrine therapy(PMID:20103631, 15664193). It can be detected the band between 38 kDa and 48 kDa by western blot.