Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293 cells, HeLa cells, A375 cells |
| Positive IHC detected in | human ovary tumor tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:11800 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
81567-1-RR targets FKBP10/FKBP65 in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag2814 Product name: Recombinant human FKBP10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 233-582 aa of BC016467 Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL 相同性解析による交差性が予測される生物種 |
| Full Name | FK506 binding protein 10, 65 kDa |
| Calculated molecular weight | 582 aa, 64 kDa |
| Observed molecular weight | 65-70 kDa |
| GenBank accession number | BC016467 |
| Gene Symbol | FKBP10 |
| Gene ID (NCBI) | 60681 |
| RRID | AB_2935562 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q96AY3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FKBP10/FKBP65 antibody 81567-1-RR | Download protocol |
| WB protocol for FKBP10/FKBP65 antibody 81567-1-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
