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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    WB analysis using 81567-1-RR (same clone as 81567-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    WB analysis of HeLa using 81567-1-RR (same clone as 81567-1-PBS)

    WB result of FKBP10/FKBP65 antibody (81567-1-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FKBP10/FKBP65 transfected HeLa cells. This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    IHC staining of human ovary tumor using 81567-1-RR (same clone as 81567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using FKBP10/FKBP65 Recombinant antibody (81567-1-RR)

    IHC staining of human ovary tumor using 81567-1-RR (same clone as 81567-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 81567-1-RR (FKBP10/FKBP65 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 81567-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81567-1-RR

    Affinity and Kinetic Characterization of 81567-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81567-1-RR against Human FKBP10/FKBP65 were performed. The affinity constant is 32.4 pM.

  • Featured Product
  • KD/KO Validated

FKBP10/FKBP65 Recombinant monoclonal antibody, PBS Only

FKBP10/FKBP65 Uni-rAb® Recombinant Antibody for WB, IHC, Indirect ELISA
Cat No. 81567-1-PBS
Clone No.S94

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

FKBP10, FKBP65, FKBP 65, FKBP 10, FK506-binding protein 10

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

81567-1-PBS targets FKBP10/FKBP65 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag2814

Product name: Recombinant human FKBP10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 233-582 aa of BC016467

Sequence: PFLAYGEKGYGTVIPPQASLVFHVLLIDVHNPKDAVQLETLELPPGCVRRAGAGDFMRYHYNGSLMDGTLFDSSYSRNHTYNTYIGQGYIIPGMDQGLQGACMGERRRITIPPHLAYGENGTGDKIPGSAVLIFNVHVIDFHNPADVVEIRTLSRPSETCNETTKLGDFVRYHYNCSLLDGTQLFTSHDYGAPQEATLGANKVIEGLDTGLQGMCVGERRQLIVPPHLAHGESGARGVPGSAVLLFEVELVSREDGLPTGYLFVWHKDPPANLFEDMDLNKDGEVPPEEFSTFIKAQVSEGKGRLMPGQDPEKTIGDMFQNQDRNQDGKITVDELKLKSDEDEERVHEEL

相同性解析による交差性が予測される生物種
Full Name FK506 binding protein 10, 65 kDa
Calculated molecular weight 582 aa, 64 kDa
Observed molecular weight65-70 kDa
GenBank accession numberBC016467
Gene Symbol FKBP10
Gene ID (NCBI) 60681
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ96AY3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FKBP10, also known as FKBP65, is a 65 kDa FK506 binding protein that is a member of the FKBP-type peptidyl-prolyl cis/trans isomerase family. This protein localizes to the endoplasmic reticulum and acts as a molecular chaperone and binding partner of type I collagen. FKBP65 is expressed in developing tissues and re-expressed in adult tissues following injury. As documented, FKBP10 has been detected to play important roles in the initiation and progression of cancers.

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