CoraLite® Plus 488-conjugated FH Polyclonal antibody
FH Polyclonal Antibody for IF
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF
Conjugate
CoraLite® Plus 488 Fluorescent Dye
Cat no : CL488-11375
Synonyms
Validation Data Gallery
Applications:
Tested Applications
| Positive IF detected in | HeLa cells |
For other applications, we recommend the unconjugated version of this antibody, 11375-1-AP
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:50-1:500 |
| Sample-dependent, check data in validation data gallery | |
Product Information
CL488-11375 targets FH in IF applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen | FH fusion protein Ag1934 相同性解析による交差性が予測される生物種 |
| Full Name | fumarate hydratase |
| Calculated molecular weight | 510 aa, 55 kDa |
| Observed molecular weight | 45-50 kDa |
| GenBank accession number | BC003108 |
| Gene symbol | FH |
| Gene ID (NCBI) | 2271 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission maxima wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Fumarate hydratase, or fumarase, is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. It belongs to the class-II fumarase/aspartase family. This protein has 2 isoforms produced by alternative initiation.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 FH antibody CL488-11375 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
