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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO33 Polyclonal antibody (23381-1-AP)

    WB analysis using 23381-1-AP (same clone as 23381-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 23381-1-AP (FBXO33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 23381-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FBXO33 Polyclonal antibody (23381-1-AP)

    IF Staining of MCF-7 using 23381-1-AP (same clone as 23381-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FBXO33 antibody (23381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 23381-1-PBS in a different storage buffer formulation.

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FBXO33 Polyclonal antibody, PBS Only

FBXO33 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 23381-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

FBX33, F-box only protein 33, c14_5247, BMND12

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

23381-1-PBS targets FBXO33 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO33 fusion protein Ag20055 相同性解析による交差性が予測される生物種
Full Name F-box protein 33
Calculated molecular weight 555 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC068566
Gene Symbol FBXO33
Gene ID (NCBI) 254170
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6M2
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

F-box Only Protein 33 (FBXO33) is a typical member of the F-box protein family with E3 ligase function. FBXO33 can regulate the ubiquitination and solubility of polyglutamine disease proteins, potentially serving as a target for treating type 3 spinocerebellar ataxia. FBXO33 ligase interferes with YB-1-mediated functions by binding and ubiquitinating the multifunctional regulator Y-box-binding protein 1 (YB-1)/dbpB/p50, leading to proteasomal degradation (PMID: 39206900). The calculated molecular weight of FBXO33 is 63 kDa.