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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FBXO33 Polyclonal antibody (23381-1-AP)

    WB analysis using 23381-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 23381-1-AP (FBXO33 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of MCF-7 cells using FBXO33 Polyclonal antibody (23381-1-AP)

    IF Staining of MCF-7 using 23381-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using FBXO33 antibody (23381-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FBXO33 Polyclonal antibody

FBXO33 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 23381-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

FBX33, F-box only protein 33, c14_5247, BMND12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inMCF-7 cells, U2OS cells, mouse lung tissue, rat lung tissue
Positive IF/ICC detected inMCF-7 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23381-1-AP targets FBXO33 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FBXO33 fusion protein Ag20055 相同性解析による交差性が予測される生物種
Full Name F-box protein 33
Calculated molecular weight 555 aa, 63 kDa
Observed molecular weight63 kDa
GenBank accession numberBC068566
Gene Symbol FBXO33
Gene ID (NCBI) 254170
RRIDAB_3669413
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ7Z6M2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

F-box Only Protein 33 (FBXO33) is a typical member of the F-box protein family with E3 ligase function. FBXO33 can regulate the ubiquitination and solubility of polyglutamine disease proteins, potentially serving as a target for treating type 3 spinocerebellar ataxia. FBXO33 ligase interferes with YB-1-mediated functions by binding and ubiquitinating the multifunctional regulator Y-box-binding protein 1 (YB-1)/dbpB/p50, leading to proteasomal degradation (PMID: 39206900). The calculated molecular weight of FBXO33 is 63 kDa.

Protocols

Product Specific Protocols
WB protocol for FBXO33 antibody 23381-1-APDownload protocol
IF protocol for FBXO33 antibody 23381-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols