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Validation Data Gallery

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  • Western Blot (WB) analysis of NCI-H1299 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of NCI-H1299 using 68246-1-Ig (same clone as 68246-1-PBS)

    NCI-H1299 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis of A549 using 68246-1-Ig (same clone as 68246-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FBXO17 Monoclonal antibody (68246-1-Ig)

    WB analysis using 68246-1-Ig (same clone as 68246-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 68246-1-Ig (FBXO17 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FBXO17 Monoclonal antibody (68246-1-Ig)

    IF Staining of HepG2 using 68246-1-Ig (same clone as 68246-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FBXO17 antibody (68246-1-Ig, Clone: 1F3D3 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68246-1-PBS in a different storage buffer formulation.

FBXO17 Monoclonal antibody, PBS Only

FBXO17 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68246-1-PBS
Clone No.1F3D3

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

F box only protein 17, F box only protein 26, F box protein 17, FBG4, Fbx17, FBX26, FBXO17, FBXO26, FLJ11798, FLJ25205, FLJ58300

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68246-1-PBS targets FBXO17 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag3867

Product name: Recombinant human FBXO17 protein

Source: e coli.-derived, T-HIS

Tag: 6*His

Domain: 1-278 aa of BC012385

Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS

相同性解析による交差性が予測される生物種
Full Name F-box protein 17
Calculated molecular weight 278 aa, 31 kDa
Observed molecular weight29-31 kDa
GenBank accession numberBC012385
Gene Symbol FBXO17
Gene ID (NCBI) 115290
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ96EF6
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.

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