Validation Data Gallery
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Tested Applications
| Positive WB detected in | NCI-H1299 cells, U2OS cells, A549 cells, SH-SY5Y cells, U-87 MG cells |
| Positive IF/ICC detected in | HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68246-1-Ig targets FBXO17 in WB, IF/ICC, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3867 Product name: Recombinant human FBXO17 protein Source: e coli.-derived, T-HIS Tag: 6*His Domain: 1-278 aa of BC012385 Sequence: MGARLSRRRLPADPSLALDALPPELLVQVLSHVPPRSLVTRCRPVCRAWRDIVDGPTVWLLQLARDRSAEGRALYAVAQRCLPSNEDKEEFPLCALARYCLRAPFGRNLIFNSCGEQGFRGWEVEHGGNGWAIEKNLTPVPGAPSQTCFVTSFEWCSKRQLVDLVMEGVWQELLDSAQIEICVADWWGARENCGCVYQLRVRLLDVYEKEVVKFSASPDPVLQWTERGCRQVSHVFTNFGKGIRYVSFEQYGRDVSSWVGHYGALVTHSSVRVRIRLS 相同性解析による交差性が予測される生物種 |
| Full Name | F-box protein 17 |
| Calculated molecular weight | 278 aa, 31 kDa |
| Observed molecular weight | 29-31 kDa |
| GenBank accession number | BC012385 |
| Gene Symbol | FBXO17 |
| Gene ID (NCBI) | 115290 |
| RRID | AB_2935332 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q96EF6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FBXO17, also named as FBG4, FBX17, FBX26 and FBXO26, is a substrate-recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FBXO17 antibody 68246-1-Ig | Download protocol |
| WB protocol for FBXO17 antibody 68246-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
