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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FASN Polyclonal antibody (10624-2-AP)

    WB analysis using 10624-2-AP (same clone as 10624-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FASN Polyclonal antibody (10624-2-AP)

    WB analysis using 10624-2-AP (same clone as 10624-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using FASN Polyclonal antibody (10624-2-AP)

    WB analysis of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of mouse liver tissue using FASN Polyclonal antibody (10624-2-AP)

    IP experiment of mouse liver using 10624-2-AP (same clone as 10624-2-PBS)

    IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:10000) with mouse liver tissue lysate 2400 ug. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using FASN Polyclonal antibody (10624-2-AP)

    IHC staining of human breast cancer using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brown adipose tissue using FASN Polyclonal antibody (10624-2-AP)

    IHC staining of mouse brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat brown adipose tissue using FASN Polyclonal antibody (10624-2-AP)

    IHC staining of rat brown adipose using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse brain tissue using FASN Polyclonal antibody (10624-2-AP)

    IHC staining of mouse brain using 10624-2-AP (same clone as 10624-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FASN Polyclonal antibody (10624-2-AP)

    IF Staining of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of HeLa cells using FASN Polyclonal antibody (10624-2-AP)

    FC experiment of HeLa using 10624-2-AP (same clone as 10624-2-PBS)

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 10624-2-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FASN Polyclonal antibody, PBS Only

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA
Cat No. 10624-2-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Synonyms

FAS, fatty acid synthase, Type I fatty acid synthase

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

10624-2-PBS targets FASN in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag0975

Product name: Recombinant human FASN protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 142-439 aa of BC007909

Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG

相同性解析による交差性が予測される生物種
Full Name fatty acid synthase
Calculated molecular weight 272 kDa
Observed molecular weight 250-272 kDa
GenBank accession numberBC007909
Gene Symbol FASN
Gene ID (NCBI) 2194
RRIDAB_2100801
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP49327
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

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