Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 10624-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis using 10624-2-AP
Various lysates were subjected to SDS PAGE followed by western blot with 10624-2-AP (FASN antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 10624-2-AP
WB result of FASN antibody (10624-2-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FASN transfected HeLa cells.
IP experiment of mouse liver using 10624-2-AP
IP result of anti-FASN (IP:10624-2-AP, 4ug; Detection:10624-2-AP 1:10000) with mouse liver tissue lysate 2400 ug.
IHC staining of human breast cancer using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brown adipose using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat brown adipose using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded rat brown adipose tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse brain using 10624-2-AP
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10624-2-AP (FASN antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 10624-2-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FASN antibody (10624-2-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HeLa using 10624-2-AP
1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human FASN (10624-2-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

FASN Polyclonal antibody

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 10624-2-AP

Publications(440)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (6)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

FAS, fatty acid synthase, Type I fatty acid synthase

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488 CoraLite® Plus 647

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (440)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HEK-293 cells, HeLa cells, HepG2 cells, SH-SY5Y cells, mouse liver tissue, rat liver tissue
Positive IP detected in	mouse liver tissue
Positive IHC detected in	human breast cancer tissue, mouse brain tissue, mouse brown adipose tissue, rat brown adipose tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells
Positive FC (Intra) detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 18 publications below
WB	See 373 publications below
IHC	See 53 publications below
IF	See 40 publications below
IP	See 13 publications below
ELISA	See 1 publications below
CoIP	See 4 publications below

Product Information

10624-2-AP targets FASN in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, rat, pig, monkey, chicken, zebrafish, sheep, goat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag0975 Product name: Recombinant human FASN protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 142-439 aa of BC007909 Sequence: QTQLNLRSLLVNPEGPTLMRLNSVQSSERPLFLVHPIEGSTTVFHSLASRLSIPTYGLQCTRAAPLDSIHSLAAYYIDCIRQVQPEGPYRVAGYSYGACVAFEMCSQLQAQQSPAPTHNSLFLFDGSPTYVLAYTQSYRAKLTPGCEAEAETEAICFFVQQFTDMEHNRVLEALLPLKGLEERVAAAVDLIIKSHQGLDRQELSFAARSFYYKLRAAEQYTPKAKYHGNVMLLRAKTGGAYGEDLGADYNLSQVCDGKVSVHVIEGDHRTLLEGSGLESIISIIHSSLAEPRVSVREG 相同性解析による交差性が予測される生物種
Full Name	fatty acid synthase
Calculated molecular weight	272 kDa
Observed molecular weight	250-272 kDa
GenBank accession number	BC007909
Gene Symbol	FASN
Gene ID (NCBI)	2194
RRID	AB_2100801
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P49327
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

FASN gene codes for an enzyme essential for de novo fatty acid synthesis and cellular substrate energy metabolism. Active FASN is a homodimer in which each peptide subunit has a molecular weight of 260 kDa. FASN is overexpressed in various types of cancer including glioblastomas and is a potential therapeutic target. Recently FASN has been reported to contribute to the neurogenesis since FASN mutation caused intellectual disability in mice.

Protocols

Product Specific Protocols
FC protocol for FASN antibody 10624-2-AP	Download protocol
IF protocol for FASN antibody 10624-2-AP	Download protocol
IHC protocol for FASN antibody 10624-2-AP	Download protocol
IP protocol for FASN antibody 10624-2-AP	Download protocol
WB protocol for FASN antibody 10624-2-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Signal Transduct Target Ther Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma. Authors - Panpan Zhang View Article
human	WB,IP	Nature Peroxisomal metabolism of branched fatty acids regulates energy homeostasis Authors - Xuejing Liu View Article
		Cancer Cell Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma. Authors - Flavia Bernardi View Article
human,mouse	WB	Nature Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy. Authors - Yifan Zhang View Article
mouse	WB	Nature Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion Authors - Ju-Qiong Wang View Article
human, mouse	WB	Nature CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis. Authors - Lingzhi Wu View Article

Validation Data Gallery

WB analysis using 10624-2-AP

WB analysis using 10624-2-AP

WB analysis of HeLa using 10624-2-AP

IP experiment of mouse liver using 10624-2-AP

IHC staining of human breast cancer using 10624-2-AP

IHC staining of mouse brown adipose using 10624-2-AP

IHC staining of rat brown adipose using 10624-2-AP

IHC staining of mouse brain using 10624-2-AP

IF Staining of HeLa using 10624-2-AP

FC experiment of HeLa using 10624-2-AP

FASN Polyclonal antibody

FASN Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

Protein C receptor maintains cancer stem cell properties via activating lipid synthesis in nasopharyngeal carcinoma.

Peroxisomal metabolism of branched fatty acids regulates energy homeostasis

Multiomic integration reveals tumoral heterogeneity of lipid dependence within lethal group 3 medulloblastoma.

Cytosolic acetyl-coenzyme A is a signalling metabolite to control mitophagy.

Inhibition of ASGR1 decreases lipid levels by promoting cholesterol excretion

CLCC1 governs ER bilayer equilibration to maintain lipid homeostasis.

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