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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARP1 Recombinant antibody (85638-4-RR)

    WB analysis using 85638-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FARP1 Recombinant antibody (85638-4-RR)

    WB analysis using 85638-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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FARP1 Recombinant antibody

FARP1 Uni-rAbTM Recombinant Antibody for WB, ELISA
Cat No. 85638-4-RR
Clone No.243132G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CDEP, Chondrocyte-derived ezrin-like protein, FERM, ARHGEF and pleckstrin domain-containing protein 1, FERM, RhoGEF and pleckstrin domain-containing protein 1, PH domain-containing family C member 2

Formulation:  PBS and Azide
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHepG2 cells, A549 cells, HeLa cells, A375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

85638-4-RR targets FARP1 in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FARP1 fusion protein Ag21225 相同性解析による交差性が予測される生物種
Full Name FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
Calculated molecular weight 1076 aa, 122 kDa
Observed molecular weight122-130 kDa
GenBank accession numberBC071592
Gene Symbol FARP1
Gene ID (NCBI) 10160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4F1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.

Protocols

Product Specific Protocols
WB protocol for FARP1 antibody 85638-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols