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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FARP1 Recombinant antibody (85638-4-RR)

    WB analysis using 85638-4-RR (same clone as 85638-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85638-4-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FARP1 Recombinant antibody (85638-4-RR)

    WB analysis using 85638-4-RR (same clone as 85638-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85638-4-RR (FARP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85638-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85638-4-RR

    Affinity and Kinetic Characterization of 85638-4-RR

    Biolayer interferometry (BLl) kinetic assays of 85638-4-RR against Human FARP1 were performed. The affinity constant is 1.18 nM.

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FARP1 Recombinant antibody, PBS Only

FARP1 Uni-rAbTM Recombinant Antibody for WB, Indirect ELISA
Cat No. 85638-4-PBS
Clone No.243132G6

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, Indirect ELISA

Synonyms

CDEP, Chondrocyte-derived ezrin-like protein, FERM, ARHGEF and pleckstrin domain-containing protein 1, FERM, RhoGEF and pleckstrin domain-containing protein 1, PH domain-containing family C member 2

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

85638-4-PBS targets FARP1 in WB, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen FARP1 fusion protein Ag21225 相同性解析による交差性が予測される生物種
Full Name FERM, RhoGEF (ARHGEF) and pleckstrin domain protein 1 (chondrocyte-derived)
Calculated molecular weight 1076 aa, 122 kDa
Observed molecular weight122-130 kDa
GenBank accession numberBC071592
Gene Symbol FARP1
Gene ID (NCBI) 10160
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ9Y4F1
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FARP1 is a protein containing a FERM (4.2, exrin, radixin, moesin) domain, a Dbl homology domain, and two pleckstrin homology domains. These domains are found in guanine nucleotide exchange factors and proteins that link the cytoskeleton to the cell membrane. The encoded protein functions in neurons to promote dendritic growth.