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Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    WB analysis of U-87 MG using 84018-4-RR (same clone as 84018-4-PBS)

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    IF Staining of Transfected HEK-293 using 84018-4-RR (same clone as 84018-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using FAP antibody (84018-4-RR, Clone: 241129B4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of Transfected HEK-293T using 84018-4-RR (same clone as 84018-4-PBS)

    1x10^6 HEK-293T cells or FAP-transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone: 241129B4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of Transfected HEK-293T using 84018-4-RR (same clone as 84018-4-PBS)

    1x10^6 untransfected (left) or FAP transfected (right) HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). 1x10^6 HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red), or Isotype Control (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of U-87 MG cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of U-87 MG using 84018-4-RR (same clone as 84018-4-PBS)

    1x10^6 U-87 MG cells were surface stained with 0.25 ug FAP Recombinant monoclonal antibody (84018-4-RR, Clone:241129B4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were not fixed. This data was developed using the same antibody clone with 84018-4-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84018-4-RR

    Affinity and Kinetic Characterization of 84018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

FAP Recombinant monoclonal antibody, PBS Only

FAP Uni-rAb® Recombinant Antibody for WB, FC, Indirect ELISA
Cat No. 84018-4-PBS
Clone No.241129B4

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, FC, Indirect ELISA

Synonyms

FAPA, 170 kDa melanoma membrane-bound gelatinase, 241129B4, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

84018-4-PBS targets FAP in WB, FC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0821

Product name: Recombinant Human FAP protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 26-760 aa of NM_004460.4

Sequence: LRPSRVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD

相同性解析による交差性が予測される生物種
Full Name fibroblast activation protein, alpha
Calculated molecular weight88 kDa
Observed molecular weight 88 kDa
GenBank accession numberNM_004460.4
Gene Symbol FAP
Gene ID (NCBI) 2191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ12884-1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

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