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Validation Data Gallery

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  • Western Blot (WB) analysis of U-87 MG cells using FAP Recombinant antibody (84018-4-RR)

    WB analysis of U-87 MG using 84018-4-RR

    U-87 MG cells were subjected to SDS PAGE followed by western blot with 84018-4-RR (FAP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of Transfected HEK-293 cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    IF Staining of Transfected HEK-293 using 84018-4-RR

    Immunofluorescent analysis of (4% PFA) fixed Transfected HEK-293 cells using FAP antibody (84018-4-RR, Clone: 241129B4 ) at dilution of 1:400 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of Transfected HEK-293T using 84018-4-RR

    1x10^6 HEK-293T cells or FAP-transfected HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone: 241129B4) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

  • Flow cytometry (FC) experiment of Transfected HEK-293T cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of Transfected HEK-293T using 84018-4-RR

    1x10^6 untransfected (left) or FAP transfected (right) HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red) or Isotype Control (blue), and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). 1x10^6 HEK-293T cells were surface stained with 0.25 ug FAP Recombinant antibody (84018-4-RR, Clone:241129B4) (red), or Isotype Control (blue) and Multi-rAb CoraLite ® Plus 647-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR005). Cells were not fixed.

  • Flow cytometry (FC) experiment of U-87 MG cells using FAP Recombinant monoclonal antibody (84018-4-RR)

    FC experiment of U-87 MG using 84018-4-RR

    1x10^6 U-87 MG cells were surface stained with 0.25 ug FAP Recombinant monoclonal antibody (84018-4-RR, Clone:241129B4) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Cells were not fixed.

  • Affinity and Kinetic Characterization of 84018-4-RR

    Affinity and Kinetic Characterization of 84018-4-RR

    Biolayer interferometry (BLl) kinetic assays of 84018-4-RR against Human FAP were performed. The affinity constant is 0.126 nM.

FAP Recombinant monoclonal antibody

FAP Uni-rAb® Recombinant Antibody for WB, FC, ELISA
Cat No. 84018-4-RR
Clone No.241129B4
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IF, FC, ELISA

Synonyms

FAPA, 170 kDa melanoma membrane-bound gelatinase, 241129B4, Antiplasmin-cleaving enzyme FAP, soluble form, Dipeptidyl peptidase FAP

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Positive WB detected inU-87 MG cells
Positive FC detected inTransfected HEK-293T cells, U-87 MG cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:8000
Flow Cytometry (FC)FC : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

IFSee 1 publications below

Product Information

84018-4-RR targets FAP in WB, IF, FC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivitymouse
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg0821

Product name: Recombinant Human FAP protein (His Tag)

Source: mammalian cells-derived, pHZ-KIsec-C-6*HIS

Tag: N-6*His

Domain: 26-760 aa of NM_004460.4

Sequence: LRPSRVHNSEENTMRALTLKDILNGTFSYKTFFPNWISGQEYLHQSADNNIVLYNIETGQSYTILSNRTMKSVNASNYGLSPDRQFVYLESDYSKLWRYSYTATYYIYDLSNGEFVRGNELPRPIQYLCWSPVGSKLAYVYQNNIYLKQRPGDPPFQITFNGRENKIFNGIPDWVYEEEMLATKYALWWSPNGKFLAYAEFNDTDIPVIAYSYYGDEQYPRTINIPYPKAGAKNPVVRIFIIDTTYPAYVGPQEVPVPAMIASSDYYFSWLTWVTDERVCLQWLKRVQNVSVLSICDFREDWQTWDCPKTQEHIEESRTGWAGGFFVSTPVFSYDAISYYKIFSDKDGYKHIHYIKDTVENAIQITSGKWEAINIFRVTQDSLFYSSNEFEEYPGRRNIYRISIGSYPPSKKCVTCHLRKERCQYYTASFSDYAKYYALVCYGPGIPISTLHDGRTDQEIKILEENKELENALKNIQLPKEEIKKLEVDEITLWYKMILPPQFDRSKKYPLLIQVYGGPCSQSVRSVFAVNWISYLASKEGMVIALVDGRGTAFQGDKLLYAVYRKLGVYEVEDQITAVRKFIEMGFIDEKRIAIWGWSYGGYVSSLALASGTGLFKCGIAVAPVSSWEYYASVYTERFMGLPTKDDNLEHYKNSTVMARAEYFRNVDYLLIHGTADDNVHFQNSAQIAKALVNAQVDFQAMWYSDQNHGLSGLSTNHLYTHMTHFLKQCFSLSD

相同性解析による交差性が予測される生物種
Full Name fibroblast activation protein, alpha
Calculated molecular weight88 kDa
Observed molecular weight 88 kDa
GenBank accession numberNM_004460.4
Gene Symbol FAP
Gene ID (NCBI) 2191
RRIDAB_3671587
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ12884-1
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Fibroblast activation protein-alpha (FAP-alpha) is a cell surface glycoprotein serine protease, which can participate in extracellular matrix degradation and is involved in many cellular processes including tissue remodeling, fibrosis, wound healing, inflammation and tumor growth (PMID: 14751930; 16223769). FAP-alpha is a single transmembrane protein that locates the cytoplasm and plasma membrane. Immunohistochemical studies have shown that FAP-alpha is transiently expressed in certain normal fetal mesenchymal tissues but that normal adult tissues as well as malignant epithelial, neural, and hematopoietic cells are generally FAP-alpha-negative. However, most of the common types of epithelial cancers contain abundant FAP-alpha-reactive stromal fibroblasts (PMID: 7911242).

Protocols

Product Specific Protocols
FC protocol for FAP antibody 84018-4-RRDownload protocol
IF protocol for FAP antibody 84018-4-RRDownload protocol
WB protocol for FAP antibody 84018-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Signal Transduct Target Ther

Photodynamic gel-bombs enhance tumor penetration and downstream synergistic therapies

Authors - Xiaole Bai
View Article
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