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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using FANCI Monoclonal antibody (67304-1-Ig)

    WB analysis of HeLa using 67304-1-Ig (same clone as 67304-1-PBS)

    WB result of FANCI antibody (67304-1-Ig; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FANCI transfected HeLa cells. This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FANCI Monoclonal antibody (67304-1-Ig)

    WB analysis using 67304-1-Ig (same clone as 67304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FANCI Monoclonal antibody (67304-1-Ig)

    WB analysis using 67304-1-Ig (same clone as 67304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FANCI Monoclonal antibody (67304-1-Ig)

    WB analysis using 67304-1-Ig (same clone as 67304-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67304-1-Ig (FANCI antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using FANCI Monoclonal antibody (67304-1-Ig)

    IHC staining of human skin cancer using 67304-1-Ig (same clone as 67304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human skin cancer tissue using FANCI Monoclonal antibody (67304-1-Ig)

    IHC staining of human skin cancer using 67304-1-Ig (same clone as 67304-1-PBS)

    Immunohistochemical analysis of paraffin-embedded skin cancer slide using 67304-1-Ig (FANCI antibody) at dilution of 1:2000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67304-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FANCI Monoclonal antibody, PBS Only

FANCI Monoclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 67304-1-PBS
Clone No.1B10E6

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Fanconi anemia group I protein, Protein FACI

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

67304-1-PBS targets FANCI in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag15153

Product name: Recombinant human FANCI protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-254 aa of BC004277

Sequence: ATMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIVSEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDE

相同性解析による交差性が予測される生物種
Full Name Fanconi anemia, complementation group I
Calculated molecular weight 1328 aa, 149 kDa
Observed molecular weight 149 kDa, 142 kDa
GenBank accession numberBC004277
Gene Symbol FANCI
Gene ID (NCBI) 55215
RRIDAB_2882566
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein G purification
UNIPROT IDQ9NVI1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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