Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, A431 cells, LNCaP cells, A549 cells, HEK-293 cells, Jurkat cells, K-562 cells, Raji cells, Daudi cells |
| Positive IHC detected in | human skin cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 3 publications below |
| IHC | See 1 publications below |
Product Information
67304-1-Ig targets FANCI in WB, IHC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15153 Product name: Recombinant human FANCI protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-254 aa of BC004277 Sequence: ATMDQKILSLAAEKTADKLQEFLQTLREGDLTNLLQNQAVKGKVAGALLRAIFKGSPCSEEAGTLRRRKIYTCCIQLVESGDLQKEIVSEIIGLLMLEAHHFPGPLLVELANEFISAVREGSLVNGKSLELLPIILTALATKKENLAYGKGVLSGEECKKQLINTLCSGRWDQQYVIQLTSMFKDVPLTAEEVEFVVEKALSMFSKMNLQEIPPLVYQLLVLSSKGSRKSVLEGIIAFFSALDKQHNEEQSGDE 相同性解析による交差性が予測される生物種 |
| Full Name | Fanconi anemia, complementation group I |
| Calculated molecular weight | 1328 aa, 149 kDa |
| Observed molecular weight | 149 kDa, 142 kDa |
| GenBank accession number | BC004277 |
| Gene Symbol | FANCI |
| Gene ID (NCBI) | 55215 |
| RRID | AB_2882566 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q9NVI1 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FANCI antibody 67304-1-Ig | Download protocol |
| WB protocol for FANCI antibody 67304-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Front Immunol FANCI serve as a prognostic biomarker correlated with immune infiltrates in skin cutaneous melanoma | ||
Adv Sci (Weinh) HPV Enhances HNSCC Chemosensitization by Inhibiting SERPINB3 Expression to Disrupt the Fanconi Anemia Pathway | ||
Adv Sci (Weinh) The Compromised Fanconi Anemia Pathway in Prelamin A-Expressing Cells Contributes to Replication Stress-Induced Genomic Instability |