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Validation Data Gallery

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  • Immunoprecipitation (IP) experiment of THP-1 cells using FAM96A Polyclonal antibody (20776-1-AP)

    IP experiment of THP-1 using 20776-1-AP (same clone as 20776-1-PBS)

    IP result of anti-FAM96A (IP:20776-1-AP, 4ug; Detection:20776-1-AP 1:600) with THP-1 cells lysate 2400 ug. This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of U-937 cells using FAM96A Polyclonal antibody (20776-1-AP)

    IP experiment of U-937 using 20776-1-AP (same clone as 20776-1-PBS)

    IP result of anti-FAM96A (IP:20776-1-AP, 4ug; Detection:20776-1-AP 1:600) with U-937 cells lysate 2240 ug. This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM96A Polyclonal antibody (20776-1-AP)

    IHC staining of mouse colon using 20776-1-AP (same clone as 20776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 20776-1-AP (FAM96A antibody) at dilution of 1:100 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat colon tissue using FAM96A Polyclonal antibody (20776-1-AP)

    IHC staining of rat colon using 20776-1-AP (same clone as 20776-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 20776-1-AP (FAM96A antibody) at dilution of 1:100 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FAM96A Polyclonal antibody (20776-1-AP)

    IF Staining of A431 using 20776-1-AP (same clone as 20776-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using FAM96A antibody (20776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of U-251 cells using FAM96A Polyclonal antibody (20776-1-AP)

    IF Staining of U-251 using 20776-1-AP (same clone as 20776-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using FAM96A antibody (20776-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 20776-1-PBS in a different storage buffer formulation.

FAM96A Polyclonal antibody, PBS Only

FAM96A Polyclonal Antibody for IHC, IF/ICC, IP, Indirect ELISA
Cat No. 20776-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, IP, Indirect ELISA

Synonyms

CIA2A, CIAO2A, Cytosolic iron-sulfur assembly component 2A, MIP18 family protein FAM96A, Protein FAM96A

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20776-1-PBS targets FAM96A in IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag14770

Product name: Recombinant human FAM96A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-90 aa of BC008865

Sequence: MQRVSGLLSWTLSRVLWLSGLSEPGAARQPRIMEEKALEVYDLIRTIRDPEKPNTLEELEVVSESCVEVQEINEEEYLVIIRFTPTVPHC

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 96, member A
Calculated molecular weight 160 aa, 18 kDa
Observed molecular weight18-21 kDa
GenBank accession numberBC008865
Gene Symbol FAM96A
Gene ID (NCBI) 84191
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9H5X1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM96A (family with sequence similarity 96, member A), also called cytosolic iron-sulfur assembly component 2A (CIAO2A), a recently identified member of the cytosolic iron-sulfur (Fe/S) protein assembly machinery and regulator of cellular iron homeostasis, is a novel pro-apoptotic APAF1-binding protein facilitating the effective induction of cell death via the mitochondrial apoptosis pathway. FAM96A is substantially enriched in macrophages comprised primarily of a DUF59 domain (residues 31-157) and an N-terminal signal peptide (residues 1-27), that is expressed at low levels in tumor cells (PMID: 29399066).

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