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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM3D Recombinant antibody (86090-2-RR)

    WB analysis using 86090-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86090-2-RR (FAM3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using FAM3D Recombinant antibody (86090-2-RR)

    WB analysis using 86090-2-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 86090-2-RR (FAM3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IF Staining of mouse colon using 86090-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FAM3D antibody (86090-2-RR, Clone: 250561E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IF Staining of mouse colon using 86090-2-RR

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FAM3D antibody (86090-2-RR, Clone: 250561E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FAM3D Recombinant monoclonal antibody

FAM3D Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, ELISA
Cat No. 86090-2-RR
Clone No.250561E10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, ELISA

Synonyms

Oit1, Oncoprotein-induced protein 1, Protein EF-7, Protein FAM3D

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inmouse colon tissue, mouse stomach tissue, mouse small intestine tissue
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse colon tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

86090-2-RR targets FAM3D in WB, IHC, IF-P, ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2694

Product name: Recombinant Mouse Oit1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-223 aa of NM_146050.2

Sequence: YTSFSRKTIRLPRWLGITPKDIQTPKSKCGLSKICPNNFFAFKISSGAANVVGPSMCFEDEIIMSPVRNNVGRGLNVALVNGSTGQVMKKDSFDMYSGDPQLLLNFLTEIPDSTLVLVASYDDPGTKMNDKIKTLFSNLGSSYAKQLGFRDSWVFVGAKDLKSKSPYEQFLKNNPETNKYDGWPELLELEGCVPRKVM

相同性解析による交差性が予測される生物種
Full Name oncoprotein induced transcript 1
Calculated molecular weight25 kDa
Observed molecular weight25 kDa
GenBank accession numberNM_146050.2
Gene Symbol Oit1
Gene ID (NCBI) 18300
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP97805
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for FAM3D antibody 86090-2-RRDownload protocol
WB protocol for FAM3D antibody 86090-2-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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