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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    WB analysis using 86090-2-RR (same clone as 86090-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86090-2-RR (FAM3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    WB analysis using 86090-2-RR (same clone as 86090-2-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 86090-2-RR (FAM3D antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IHC staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 86090-2-RR (FAM3D antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IF Staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FAM3D antibody (86090-2-RR, Clone: 250561E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of mouse colon tissue using FAM3D Recombinant monoclonal antibody (86090-2-RR)

    IF Staining of mouse colon using 86090-2-RR (same clone as 86090-2-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse colon tissue using FAM3D antibody (86090-2-RR, Clone: 250561E10 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 86090-2-PBS in a different storage buffer formulation.

Mouse FAM3D Recombinant monoclonal antibody, PBS Only

FAM3D Uni-rAb® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA
Cat No. 86090-2-PBS
Clone No.250561E10

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

Oit1, Oncoprotein-induced protein 1, Protein EF-7, Protein FAM3D

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

86090-2-PBS targets FAM3D in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

Tested Reactivity mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Eg2694

Product name: Recombinant Mouse Oit1 protein (rFc Tag)

Source: mammalian cells-derived, V37

Tag: C-rFc

Domain: 26-223 aa of NM_146050.2

Sequence: YTSFSRKTIRLPRWLGITPKDIQTPKSKCGLSKICPNNFFAFKISSGAANVVGPSMCFEDEIIMSPVRNNVGRGLNVALVNGSTGQVMKKDSFDMYSGDPQLLLNFLTEIPDSTLVLVASYDDPGTKMNDKIKTLFSNLGSSYAKQLGFRDSWVFVGAKDLKSKSPYEQFLKNNPETNKYDGWPELLELEGCVPRKVM

相同性解析による交差性が予測される生物種
Full Name oncoprotein induced transcript 1
Calculated molecular weight25 kDa
Observed molecular weight25 kDa
GenBank accession numberNM_146050.2
Gene Symbol Oit1
Gene ID (NCBI) 18300
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDP97805
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.
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