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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM210A Polyclonal antibody (31694-1-AP)

    WB analysis using 31694-1-AP (same clone as 31694-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 31694-1-AP (FAM210A antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 31694-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FAM210A Polyclonal antibody (31694-1-AP)

    IHC staining of human ovary cancer using 31694-1-AP (same clone as 31694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31694-1-AP (FAM210A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31694-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human ovary cancer tissue using FAM210A Polyclonal antibody (31694-1-AP)

    IHC staining of human ovary cancer using 31694-1-AP (same clone as 31694-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 31694-1-AP (FAM210A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 31694-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using FAM210A Polyclonal antibody (31694-1-AP)

    IF Staining of A431 using 31694-1-AP (same clone as 31694-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using FAM210A antibody (31694-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 31694-1-PBS in a different storage buffer formulation.

FAM210A Polyclonal antibody, PBS Only

FAM210A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 31694-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C18orf19

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

31694-1-PBS targets FAM210A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag36161

Product name: Recombinant human C18orf19 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 157-272 aa of NM_001098801

Sequence: LKGVNVVPFLELIGLPDSVVSILKNSQSGNALTAYALFKIATPARYTVTLGGTSVTVKYLRSHGYMSTPPPVKEYLQDRMEETKELITEKMEETKDRLTEKLQETKEKVSFKKKVE

相同性解析による交差性が予測される生物種
Full Name chromosome 18 open reading frame 19
Calculated molecular weight30kDa
Observed molecular weight 20 kDa
GenBank accession numberNM_001098801
Gene Symbol C18orf19
Gene ID (NCBI) 125228
RRIDAB_3670081
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity Purification
UNIPROT IDQ96ND0
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM210A also known as C18orf19, is a determinant of bone and muscle structure and strength. FAM210A is required for cold-induced mitochondrial cristae remodeling, adaptive thermogenic activity, and brown adipose tissue characteristics(PMID: 37816711). FAM210A is localized to mitochondria and cytoplasm of muscle cells and myotubes(PMID: 29618611).

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