Validation Data Gallery
Tested Applications
| Positive WB detected in | mouse adipose tissue, mouse heart tissue, rat adipose tissue |
| Positive IHC detected in | human ovary cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A431 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
31694-1-AP targets FAM210A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag36161 Product name: Recombinant human C18orf19 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 157-272 aa of NM_001098801 Sequence: LKGVNVVPFLELIGLPDSVVSILKNSQSGNALTAYALFKIATPARYTVTLGGTSVTVKYLRSHGYMSTPPPVKEYLQDRMEETKELITEKMEETKDRLTEKLQETKEKVSFKKKVE 相同性解析による交差性が予測される生物種 |
| Full Name | chromosome 18 open reading frame 19 |
| Calculated molecular weight | 30kDa |
| Observed molecular weight | 20 kDa |
| GenBank accession number | NM_001098801 |
| Gene Symbol | C18orf19 |
| Gene ID (NCBI) | 125228 |
| RRID | AB_3670081 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity Purification |
| UNIPROT ID | Q96ND0 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM210A also known as C18orf19, is a determinant of bone and muscle structure and strength. FAM210A is required for cold-induced mitochondrial cristae remodeling, adaptive thermogenic activity, and brown adipose tissue characteristics(PMID: 37816711). FAM210A is localized to mitochondria and cytoplasm of muscle cells and myotubes(PMID: 29618611).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM210A antibody 31694-1-AP | Download protocol |
| IHC protocol for FAM210A antibody 31694-1-AP | Download protocol |
| WB protocol for FAM210A antibody 31694-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |