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Validation Data Gallery

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  • Western Blot (WB) analysis of NIH/3T3 cells using FAM129B Polyclonal antibody (22553-1-AP)

    WB analysis of NIH/3T3 using 22553-1-AP (same clone as 22553-1-PBS)

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22553-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FAM129B Polyclonal antibody (22553-1-AP)

    WB analysis using 22553-1-AP (same clone as 22553-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 22553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FAM129B Polyclonal antibody (22553-1-AP)

    IHC staining of human liver using 22553-1-AP (same clone as 22553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22553-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using FAM129B Polyclonal antibody (22553-1-AP)

    IHC staining of human liver using 22553-1-AP (same clone as 22553-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 22553-1-PBS in a different storage buffer formulation.

FAM129B Polyclonal antibody, PBS Only

FAM129B Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 22553-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

22553-1-PBS targets FAM129B in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag18314

Product name: Recombinant human FAM129B protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-341 aa of BC067366

Sequence: DARRQHIAEKTGKILTEFLQFYEDQYGVALFNSMRHEIEGTGLPQAQLLWRKVPLDERIVFSGNLFQHQEDSKKWRNRFSLVPHNYGLVLYENKAAYERQVPPRAVINSAGYKILTSVDQYLELIGNSLPGTTAKSGSAPILKCPTQFPLILWHPYARHYYFCMMTEAEQDKWQAVLQDCIRHCNNGIPEDSKVEGPAFTDAIRMYRQSKELYGTWEMLCGNEVQILSNLVMEELGPELKAELGPRLKGKPQERQRQWIQISDAVYHMVYEQAKARFEEVLSKVQQVQPAMQAVIRTDMDQIITSKEHLASKIRAFILPKAEVCVRNHVQPYIPSILEALM

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 129, member B
Calculated molecular weight 733 aa, 83 kDa
Observed molecular weight 83-93 kDa
GenBank accession numberBC067366
Gene Symbol FAM129B
Gene ID (NCBI) 64855
RRIDAB_2879121
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen Affinity purified
UNIPROT IDQ96TA1
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM129B, also named as Niban-like protein 1 or C9orf88, is a 746 amino acid protein, which contains one PH domain and belongs to the Niban family. FAM129B localizes in the cytoplasm and may play a role in apoptosis suppression and promote melanoma cell invasion in vitro.

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