• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0
×

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

FAM129B Polyclonal antibody

FAM129B Polyclonal Antibody for WB, FC, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, FC, IHC, ELISA

Conjugate

Unconjugated

Cat no : 22553-1-AP

Synonyms

C9orf88, FAM129B, MEG 3, Niban like protein 1, OC58, Protein FAM129B

Validation Data Gallery

Applications:
  • WB analysis of NIH/3T3 using 22553-1-AP

    WB analysis of NIH/3T3 using 22553-1-AP

    NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis using 22553-1-AP

    WB analysis using 22553-1-AP

    A431 cells were subjected to SDS PAGE followed by western blot with 22553-1-AP (FAM129B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human liver using 22553-1-AP

    IHC staining of human liver using 22553-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22553-1-AP (FAM129B antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • FC experiment of HeLa using 22553-1-AP

    FC experiment of HeLa using 22553-1-AP

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human FAM129B (22553-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH .

Tested Applications

Positive WB detected inNIH/3T3 cells, A431 cells
Positive IHC detected inhuman liver tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
Sample-dependent, check data in validation data gallery

Product Information

22553-1-AP targets FAM129B in WB, FC, IHC, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM129B fusion protein Ag18314 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 129, member B
Calculated molecular weight 733 aa, 83 kDa
Observed molecular weight 83-93 kDa
GenBank accession numberBC067366
Gene symbol FAM129B
Gene ID (NCBI) 64855
RRIDAB_2879121
Conjugate Unconjugated
Form Liquid
Purification Method Antigen Affinity purified
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM129B, also named as Niban-like protein 1 or C9orf88, is a 746 amino acid protein, which contains one PH domain and belongs to the Niban family. FAM129B localizes in the cytoplasm and may play a role in apoptosis suppression and promote melanoma cell invasion in vitro.

Protocols

Product Specific Protocols
WB protocol for FAM129B antibody 22553-1-APDownload protocol
IHC protocol for FAM129B antibody 22553-1-APDownload protocol
FC protocol for FAM129B antibody 22553-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols