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Validation Data Gallery

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  • Western Blot (WB) analysis of siRNA cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of siRNA cells using 21529-1-AP (same clone as 21529-1-PBS)

    WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A. This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HEK-293 cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of HEK-293 using 21529-1-AP (same clone as 21529-1-PBS)

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of HepG2 using 21529-1-AP (same clone as 21529-1-PBS)

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FAM120A Polyclonal antibody (21529-1-AP)

    IHC staining of human stomach cancer using 21529-1-AP (same clone as 21529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21529-1-AP (FAM120A antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human stomach cancer tissue using FAM120A Polyclonal antibody (21529-1-AP)

    IHC staining of human stomach cancer using 21529-1-AP (same clone as 21529-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 21529-1-AP (FAM120A antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM120A Polyclonal antibody (21529-1-AP)

    IF Staining of HepG2 using 21529-1-AP (same clone as 21529-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM120A antibody (21529-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4). This data was developed using the same antibody clone with 21529-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

FAM120A Polyclonal antibody, PBS Only

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 21529-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21529-1-PBS targets FAM120A in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16149

Product name: Recombinant human FAM120A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 285-538 aa of BC111736

Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 120A
Calculated molecular weight 1118 aa, 122 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC111736
Gene Symbol FAM120A
Gene ID (NCBI) 23196
RRIDAB_10860261
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZB2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

FAM120A (also named OSSA/C9ORF10) is mapped to chromosome 9q22.31, which was first reported to be a component of Purα-containing mRNP complexes. FAM120A as a transcription co-activator that couples transcription and splicing of de novo lipid synthesis enzymes downstream of mTORC1-serine/arginine-rich protein kinase 2 (SRPK2) signaling. FAM120A is abundantly expressed in the cytoplasm of the GC cells and elevated FAM120A is observed in the scirrhous-type GC tissues compared with normal gastric mucosa. (PMID: 38565940, PMID: 37595559)

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