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Validation Data Gallery

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  • Western Blot (WB) analysis of siRNA cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of siRNA cells using 21529-1-AP

    WB result of FAM120A antibody (21529-1-AP) with siRNA control and siRNA-FAM120A.

  • Western Blot (WB) analysis of HEK-293 cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of HEK-293 using 21529-1-AP

    HEK-293 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of HepG2 cells using FAM120A Polyclonal antibody (21529-1-AP)

    WB analysis of HepG2 using 21529-1-AP

    HepG2 cells were subjected to SDS PAGE followed by western blot with 21529-1-AP (FAM120A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using FAM120A Polyclonal antibody (21529-1-AP)

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human skeletal muscle tissue using FAM120A Polyclonal antibody (21529-1-AP)

    IHC staining of human skeletal muscle using 21529-1-AP

    Immunohistochemical analysis of paraffin-embedded human skeletal muscle using 21529-1-AP (FAM120A antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using FAM120A Polyclonal antibody (21529-1-AP)

    IF Staining of HepG2 using 21529-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using FAM120A antibody (21529-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-4).

  • Featured Product
  • KD/KO Validated

FAM120A Polyclonal antibody

FAM120A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 21529-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

C9orf10, Constitutive coactivator of PPAR-gamma-like protein 1, KIAA0183, OSSA, Oxidative stress-associated SRC activator

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293 cells, siRNA cells
Positive IHC detected inhuman skeletal muscle tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

21529-1-AP targets FAM120A in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityrat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag16149

Product name: Recombinant human FAM120A protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 285-538 aa of BC111736

Sequence: AVADYVRNIQDTSDLDAIAKDVFQHSQSRTDDKVIRFKRAIGYYSATSKPMSFHPPHYLAARPGPFGMPGMVPPHVPPQMLNIPQTSLQAKPVAPQVPSPGGAPGQGPYPYSLSEPAPLTLDTSGKNLTEQNSYSNIPHEGKHTPLYERSSPINPAQSGSPNHVDSAYFPGSSTSSSSDNDEGSGGATNHISGNKIGWEKTGSHSEPQARGDPGDQTKAEGSSTASSGSQLAEGKGSQMGTVQPIPCLLSMPTR

相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 120A
Calculated molecular weight 1118 aa, 122 kDa
Observed molecular weight 125 kDa
GenBank accession numberBC111736
Gene Symbol FAM120A
Gene ID (NCBI) 23196
RRIDAB_10860261
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NZB2
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FAM120A antibody 21529-1-APDownload protocol
IHC protocol for FAM120A antibody 21529-1-APDownload protocol
IF protocol for FAM120A antibody 21529-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
ratWB

Mol Neurobiol

Monosome Stalls the Translation Process Mediated by IGF2BP in Arcuate Nucleus for Puberty Onset Delay

Authors - Yifen Shen
View Article