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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM114A2 Polyclonal antibody (29917-1-AP)

    WB analysis using 29917-1-AP (same clone as 29917-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 29917-1-AP (FAM114A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 29917-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM114A2 Polyclonal antibody (29917-1-AP)

    IF Staining of HeLa using 29917-1-AP (same clone as 29917-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM114A2 antibody (29917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 29917-1-PBS in a different storage buffer formulation.

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FAM114A2 Polyclonal antibody, PBS Only

FAM114A2 Polyclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 29917-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

29917-1-PBS targets FAM114A2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM114A2 fusion protein Ag31963 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 114, member A2
Observed molecular weight55 kDa
GenBank accession numberBC011414
Gene Symbol FAM114A2
Gene ID (NCBI) 10827
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRY5
Storage Buffer PBS only , pH 7.3
Storage ConditionsStore at -80°C.

Background Information

FAM114A2, also known as C5orf3, belongs to the nervous overexpress protein family and has been implicated in neuronal cell development. FAM114A2 a human testis enrich gene and testis sperm-binding protein, is highly conserved in mammals. The observed band size is around 55 kDa.