Validation Data Gallery
Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells, Jurkat cells, MOLT-4 cells, U-251 cells, mouse testis tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29917-1-AP targets FAM114A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.
| Tested Reactivity | Human, Mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31963 Product name: Recombinant human FAM114A2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 255-505 aa of BC011414 Sequence: MLSQESEIKVKSILNSLSGEELETLKVELEQLKETFSLAEFCEEEEEEKKGDEDFTKDITELFSQLHVSSKPEKLARARNTAHEWIRKSLTKPLAENEEGEKQSEAENTEQVNKNSIEDIHAFAIRSLAELTACSIELFHKTAALVLHGRKQEVTAIERSQTLSQMTIVLCKELSSLSKEFTTCLTTAGVKEMADVLNPLITAVFLEASNSASYIQDAFQLLLPVLEISLIENKIESHRHELQGQKPLLEH 相同性解析による交差性が予測される生物種 |
| Full Name | family with sequence similarity 114, member A2 |
| Observed molecular weight | 55 kDa |
| GenBank accession number | BC011414 |
| Gene Symbol | FAM114A2 |
| Gene ID (NCBI) | 10827 |
| RRID | AB_3086188 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9NRY5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
FAM114A2, also known as C5orf3, belongs to the nervous overexpress protein family and has been implicated in neuronal cell development. FAM114A2 a human testis enrich gene and testis sperm-binding protein, is highly conserved in mammals. The observed band size is around 55 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FAM114A2 antibody 29917-1-AP | Download protocol |
| WB protocol for FAM114A2 antibody 29917-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
