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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FAM114A2 Polyclonal antibody (29917-1-AP)

    WB analysis using 29917-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29917-1-AP (FAM114A2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FAM114A2 Polyclonal antibody (29917-1-AP)

    IF Staining of HeLa using 29917-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using FAM114A2 antibody (29917-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

［PR］まずは試したいあなたに。トライアルサイズも特別価格 >>『PRIME BUY抗体特割キャンペーン（4/21～）』

FAM114A2 Polyclonal antibody

FAM114A2 Polyclonal Antibody for WB, IF/ICC, ELISA
Cat No. 29917-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse

Applications

WB, IF/ICC, ELISA

Synonyms

133K02, C5orf3, FAM114A2, Protein FAM114A2

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, Jurkat cells, MOLT-4 cells, U-251 cells, mouse testis tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29917-1-AP targets FAM114A2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity Human, Mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FAM114A2 fusion protein Ag31963 相同性解析による交差性が予測される生物種
Full Name family with sequence similarity 114, member A2
Observed molecular weight55 kDa
GenBank accession numberBC011414
Gene Symbol FAM114A2
Gene ID (NCBI) 10827
RRIDAB_3086188
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9NRY5
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FAM114A2, also known as C5orf3, belongs to the nervous overexpress protein family and has been implicated in neuronal cell development. FAM114A2 a human testis enrich gene and testis sperm-binding protein, is highly conserved in mammals. The observed band size is around 55 kDa.

Protocols

Product Specific Protocols
WB protocol for FAM114A2 antibody 29917-1-APDownload protocol
IF protocol for FAM114A2 antibody 29917-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols