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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using FADD Polyclonal antibody (14906-1-AP)

    WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of mouse pancreas tissue using FADD Polyclonal antibody (14906-1-AP)

    WB analysis of mouse pancreas using 14906-1-AP (same clone as 14906-1-PBS)

    mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of A549 cells using FADD Polyclonal antibody (14906-1-AP)

    WB analysis of A549 using 14906-1-AP (same clone as 14906-1-PBS)

    A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using FADD Polyclonal antibody (14906-1-AP)

    WB analysis using 14906-1-AP (same clone as 14906-1-PBS)

    mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of A549 cells using FADD Polyclonal antibody (14906-1-AP)

    IP experiment of A549 using 14906-1-AP (same clone as 14906-1-PBS)

    IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug. This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon tissue using FADD Polyclonal antibody (14906-1-AP)

    IHC staining of human colon using 14906-1-AP (same clone as 14906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human cervical cancer tissue using FADD Polyclonal antibody (14906-1-AP)

    IHC staining of human cervical cancer using 14906-1-AP (same clone as 14906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using FADD Polyclonal antibody (14906-1-AP)

    IHC staining of human lung cancer using 14906-1-AP (same clone as 14906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of rat kidney tissue using FADD Polyclonal antibody (14906-1-AP)

    IHC staining of rat kidney using 14906-1-AP (same clone as 14906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of mouse kidney tissue using FADD Polyclonal antibody (14906-1-AP)

    IHC staining of mouse kidney using 14906-1-AP (same clone as 14906-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using FADD Polyclonal antibody (14906-1-AP)

    IF Staining of HeLa using 14906-1-AP (same clone as 14906-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 14906-1-PBS in a different storage buffer formulation.

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FADD Polyclonal antibody, PBS Only

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 14906-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein

Formulation:  PBS Only
PBS and Azide
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14906-1-PBS targets FADD in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FADD fusion protein Ag6701 相同性解析による交差性が予測される生物種
Full Name Fas (TNFRSF6)-associated via death domain
Calculated molecular weight 23 kDa
Observed molecular weight 23-30 kDa
GenBank accession numberBC000334
Gene Symbol FADD
Gene ID (NCBI) 8772
RRIDAB_2100486
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDQ13158
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)