Validation Data Gallery

Filter: AllWBIPIHCIF/ICC

WB analysis using 14906-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of mouse pancreas using 14906-1-AP
mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.
WB analysis of A549 using 14906-1-AP
A549 cells were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis using 14906-1-AP
mouse bone marrow lysates were subjected to SDS PAGE followed by western blot with 14906-1-AP (FADD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.
IP experiment of A549 using 14906-1-AP
IP result of anti-FADD (IP:14906-1-AP, 4ug; Detection:14906-1-AP 1:300) with A549 cells lysate 3040ug.
IHC staining of human colon using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human lung cancer using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of mouse kidney using 14906-1-AP
Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 14906-1-AP (FADD antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HeLa using 14906-1-AP
Immunofluorescent analysis of (4% PFA) fixed HeLa cells using FADD antibody (14906-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

FADD Polyclonal antibody

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 14906-1-AP

Publications(67)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (4)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Synonyms

MORT1, Growth-inhibiting gene 3 protein, FAS-associating death domain-containing protein, FAS-associated death domain protein

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (67)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	HT-1080 cells, mouse pancreas tissue, A549 cells, mouse bone marrow, HeLa cells, HepG2 cells, Jurkat cells
Positive IP detected in	A549 cells
Positive IHC detected in	human lung cancer tissue, human colon tissue, human cervical cancer tissue, rat kidney tissue, mouse kidney tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	HeLa cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 2 publications below
WB	See 56 publications below
IHC	See 8 publications below
IF	See 1 publications below
IP	See 6 publications below
CoIP	See 2 publications below

Product Information

14906-1-AP targets FADD in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity	human, mouse
Cited Reactivity	human, mouse, rat, monkey, bovine, sheep
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6701 Product name: Recombinant human FADD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-208 aa of BC000334 Sequence: MDPFLVLLHSVSSSLSSSELTELKFLCLGRVGKRKLERVQSGLDLFSMLLEQNDLEPGHTELLRELLASLRRHDLLRRVDDFEAGAAAGAAPGEEDLCAAFNVICDNVGKDWRRLARQLKVSDTKIDSIEDRYPRNLTERVRESLRIWKNTEKENATVAHLVGALRSCQMNLVADLVQEVQQARDLQNRSGAMSPMSWNSDASTSEAS 相同性解析による交差性が予測される生物種
Full Name	Fas (TNFRSF6)-associated via death domain
Calculated molecular weight	23 kDa
Observed molecular weight	23-30 kDa
GenBank accession number	BC000334
Gene Symbol	FADD
Gene ID (NCBI)	8772
RRID	AB_2100486
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q13158
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

Fas-Associated protein with Death Domain (FADD), also called MORT1 or GIG3, is encoded by the FADD gene. FADD is an adaptor protein that bridges members of the tumor necrosis factor receptor superfamily, such as the Fas-receptor, to procaspases 8 and 10 to form the death-inducing signaling complex (DISC) during apoptosis. As well as its most well known role in apoptosis, FADD has also been seen to play a role in other processes including proliferation, cell cycle regulation and development. FADD has a calculated molecular mass of 23 kDa and always can be detected as 23-30 kDa (PMID: 15390286, 22864571, 17977957)

Protocols

Product Specific Protocols
IF protocol for FADD antibody 14906-1-AP	Download protocol
IHC protocol for FADD antibody 14906-1-AP	Download protocol
IP protocol for FADD antibody 14906-1-AP	Download protocol
WB protocol for FADD antibody 14906-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
human	WB	Cell Res The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8. Authors - Jia-Yuan Zhang View Article
human	IP,WB	Nat Immunol Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency Authors - Hirotsugu Oda View Article
		Blood Expression of cFLIP in B cells is essential for diffuse large B-cell lymphoma pathogenesis. Authors - Kristie Tanavura Bariboloka View Article
human	WB	J Exp Clin Cancer Res Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells. Authors - Wentao An View Article
human	WB,CoIP	Cancer Biol Med A causal variant rs3769823 in 2q33.1 involved in apoptosis pathway leading to a decreased risk of non-small cell lung cancer Authors - Xu Zhang View Article
		Pharmacol Res Study on epigenetic regulatory mechanism of MBD4 with a germline loss-of-function mutation (rs140693) responsible for retinoblastoma chemoresistance via the cell cycle and apoptotic pathways. Authors - Li Zhang View Article

Validation Data Gallery

WB analysis using 14906-1-AP

WB analysis of mouse pancreas using 14906-1-AP

WB analysis of A549 using 14906-1-AP

WB analysis using 14906-1-AP

IP experiment of A549 using 14906-1-AP

IHC staining of human colon using 14906-1-AP

IHC staining of human cervical cancer using 14906-1-AP

IHC staining of human lung cancer using 14906-1-AP

IHC staining of rat kidney using 14906-1-AP

IHC staining of mouse kidney using 14906-1-AP

IF Staining of HeLa using 14906-1-AP

FADD Polyclonal antibody

FADD Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

The metabolite α-KG induces GSDMC-dependent pyroptosis through death receptor 6-activated caspase-8.

Biallelic human SHARPIN loss of function induces autoinflammation and immunodeficiency

Expression of cFLIP in B cells is essential for diffuse large B-cell lymphoma pathogenesis.

Glucocorticoid modulatory element-binding protein 1 (GMEB1) interacts with the de-ubiquitinase USP40 to stabilize CFLARL and inhibit apoptosis in human non-small cell lung cancer cells.

A causal variant rs3769823 in 2q33.1 involved in apoptosis pathway leading to a decreased risk of non-small cell lung cancer

Study on epigenetic regulatory mechanism of MBD4 with a germline loss-of-function mutation (rs140693) responsible for retinoblastoma chemoresistance via the cell cycle and apoptotic pathways.

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