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Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using EYA3 Polyclonal antibody (21196-1-AP)

    WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using EYA3 Polyclonal antibody (21196-1-AP)

    WB analysis of L02 using 21196-1-AP (same clone as 21196-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of L02 cells using EYA3 Polyclonal antibody (21196-1-AP)

    WB analysis of L02 using 21196-1-AP (same clone as 21196-1-PBS)

    L02 cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EYA3 Polyclonal antibody (21196-1-AP)

    WB analysis of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 21196-1-AP (EYA3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunoprecipitation (IP) experiment of L02 cells using EYA3 Polyclonal antibody (21196-1-AP)

    IP experiment of L02 using 21196-1-AP (same clone as 21196-1-PBS)

    IP result of anti-EYA3 (IP:21196-1-AP, 4ug; Detection:21196-1-AP 1:1000) with L02 cells lysate 1800ug. This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EYA3 Polyclonal antibody (21196-1-AP)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EYA3 Polyclonal antibody (21196-1-AP)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EYA3 Polyclonal antibody (21196-1-AP)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using EYA3 Polyclonal antibody (21196-1-AP)

    IHC staining of human breast cancer using 21196-1-AP (same clone as 21196-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21196-1-AP (EYA3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EYA3 Polyclonal antibody (21196-1-AP)

    IF Staining of HeLa using 21196-1-AP (same clone as 21196-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using EYA3 antibody (21196-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Flow cytometry (FC) experiment of A431 cells using EYA3 Polyclonal antibody (21196-1-AP)

    FC experiment of A431 using 21196-1-AP (same clone as 21196-1-PBS)

    1x10^6 A431 cells were intracellularly stained with 0.25 ug EYA3 Polyclonal antibody (21196-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 21196-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EYA3 Polyclonal antibody, PBS Only

EYA3 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA
Cat No. 21196-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

EC:3.1.3.48

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

21196-1-PBS targets EYA3 in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag15544

Product name: Recombinant human EYA3 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-300 aa of BC029500

Sequence: MEEEQDLPEQPVKKAKMQESGEQTISQVSNPDVSDQKPETSSLASNLPMSEEIMTCTDYIPRSSNDYTSQMYSAKPYAHILSVPVSETAYPGQTQYQTLQQTQPYAVYPQATQTYGLPPFGALWPGMKPESGLIQTPSPSQHSVLTCTTGLTTSQPSPAHYSYPIQASSTNASLISTSSTIANIPAAAVASISNQDYPTYTILGQNQYQACYPSSSFGVTGQTNSDAESTTLAATTYQSEKPSVMAPAPAAQRLSSGDPSTSPSLSQTTPSKDTDDQSRKNMTSKNRGKRKADATSSQDS

相同性解析による交差性が予測される生物種
Full Name eyes absent homolog 3 (Drosophila)
Calculated molecular weight 573 aa, 63 kDa
Observed molecular weight 63-66 kDa
GenBank accession numberBC029500
Gene Symbol EYA3
Gene ID (NCBI) 2140
RRIDAB_10732957
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ99504
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EYA3(Eyes absent homolog 3) belongs to the HAD-like hydrolase superfamily.Its function as histone phosphatase probably explains its role in transcription regulation during organogenesis.EYA3, like EYA1, is expressed in the ninth week of human development and may also underlie developmental defects(PMID:9020840).It has 2 isoforms produced by alternative splicing.This antibody is specific to EYA3.

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