Validation Data Gallery
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Tested Applications
| Positive WB detected in | HeLa cells, MCF-7 cells, Jurkat cells, SGC-7901 cells, HEK-293T cells, rat skin tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
83875-5-RR targets EXOSC1 in WB, ELISA applications and shows reactivity with human, rat samples.
| Tested Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
CatNo: Ag3286 Product name: Recombinant human EXOSC1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-195 aa of BC022067 Sequence: MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT 相同性解析による交差性が予測される生物種 |
| Full Name | exosome component 1 |
| Calculated molecular weight | 195 aa, 21 kDa |
| Observed molecular weight | 21 kDa |
| GenBank accession number | BC022067 |
| Gene Symbol | EXOSC1 |
| Gene ID (NCBI) | 51013 |
| RRID | AB_3671460 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purfication |
| UNIPROT ID | Q9Y3B2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for EXOSC1 antibody 83875-5-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |