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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EXOSC1 Recombinant antibody (83875-5-RR)

    WB analysis using 83875-5-RR (same clone as 83875-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83875-5-RR (EXOSC1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83875-5-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of MCF-7 cells using EXOSC1 Recombinant antibody (83875-5-RR)

    WB analysis of MCF-7 using 83875-5-RR (same clone as 83875-5-PBS)

    WB result of EXOSC1 antibody (83875-5-RR; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EXOSC1 transfected MCF-7 cells. This data was developed using the same antibody clone with 83875-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83875-5-RR

    Affinity and Kinetic Characterization of 83875-5-RR

    Biolayer interferometry (BLl) kinetic assays of 83875-5-RR against Human EXOSC1 were performed. The affinity constant is 1.86 nM.

  • Featured Product
  • KD/KO Validated

EXOSC1 Recombinant monoclonal antibody, PBS Only

EXOSC1 Uni-rAb® Recombinant Antibody for WB, Indirect ELISA
Cat No. 83875-5-PBS
Clone No.240938C6

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Indirect ELISA

Synonyms

Exosome component 1, Exosome complex component CSL4, Csl4p, CSL4, CGI-108

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

83875-5-PBS targets EXOSC1 in WB, Indirect ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity human, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen

CatNo: Ag3286

Product name: Recombinant human EXOSC1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-195 aa of BC022067

Sequence: MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAIVTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQSNYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT

相同性解析による交差性が予測される生物種
Full Name exosome component 1
Calculated molecular weight 195 aa, 21 kDa
Observed molecular weight21 kDa
GenBank accession numberBC022067
Gene Symbol EXOSC1
Gene ID (NCBI) 51013
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purfication
UNIPROT IDQ9Y3B2
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EXOSC1 is a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening.

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