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Validation Data Gallery

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  • Western Blot (WB) analysis of BGC-823 cells using ETHE1 Polyclonal antibody (27786-1-AP)

    WB analysis of BGC-823 using 27786-1-AP (same clone as 27786-1-PBS)

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using ETHE1 Polyclonal antibody (27786-1-AP)

    WB analysis using 27786-1-AP (same clone as 27786-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human liver using 27786-1-AP (same clone as 27786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human liver tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human liver using 27786-1-AP (same clone as 27786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human colon cancer using 27786-1-AP (same clone as 27786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human colon cancer using 27786-1-AP (same clone as 27786-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 27786-1-PBS in a different storage buffer formulation.

ETHE1 Polyclonal antibody, PBS Only

ETHE1 Polyclonal Antibody for WB, IHC, Indirect ELISA
Cat No. 27786-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

27786-1-PBS targets ETHE1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag26597

Product name: Recombinant human ETHE1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 8-132 aa of BC008250

Sequence: VARRQLSQRGGSGAPILLRQMFEPVSCTFTYLLGDRESREAVLIDPVLETAPRDAQLIKELGLRLLYAVNTHCHADHITGSGLLRSLLPGCQSVISRLSGAQADLHIEDGDSIRFGRFALETRAS

相同性解析による交差性が予測される生物種
Full Name ethylmalonic encephalopathy 1
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC008250
Gene Symbol ETHE1
Gene ID (NCBI) 23474
RRIDAB_2880971
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDO95571
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

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