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Validation Data Gallery

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  • Western Blot (WB) analysis of BGC-823 cells using ETHE1 Polyclonal antibody (27786-1-AP)

    WB analysis of BGC-823 using 27786-1-AP

    BGC-823 cells were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using ETHE1 Polyclonal antibody (27786-1-AP)

    WB analysis using 27786-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 27786-1-AP (ETHE1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human liver using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human colon cancer using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human colon cancer tissue using ETHE1 Polyclonal antibody (27786-1-AP)

    IHC staining of human colon cancer using 27786-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 27786-1-AP (ETHE1 antibody) at dilution of 1:200 (under 10x lens).

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ETHE1 Polyclonal antibody

ETHE1 Polyclonal Antibody for WB, IHC, ELISA
Cat No. 27786-1-AP
Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse and More (1)

Applications

WB, IHC, ELISA

Synonyms

ETHE1, ethylmalonic encephalopathy 1, HSCO, Protein ETHE1, mitochondrial, YF13H12

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

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保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inBGC-823 cells, HeLa cells, mouse liver tissue
Positive IHC detected inhuman liver tissue, human colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27786-1-AP targets ETHE1 in WB, IHC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Reactivity Human, mouse
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen ETHE1 fusion protein Ag26597 相同性解析による交差性が予測される生物種
Full Name ethylmalonic encephalopathy 1
Calculated molecular weight 28 kDa
Observed molecular weight 28 kDa
GenBank accession numberBC008250
Gene Symbol ETHE1
Gene ID (NCBI) 23474
RRIDAB_2880971
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95571
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

ETHE1, a gene encoding a b-lactamase-like, iron-coordinating metalloprotein, is mutated in ethylmalonic encephalopathy.In humans, ETHE1 gene is proved to encode the SDO in mitochondria and its mutations lead to a fatal autosomal recessive mitochondrial disease: ethylmalonic encephalopathy.

Protocols

Product Specific Protocols
WB protocol for ETHE1 antibody 27786-1-APDownload protocol
IHC protocol for ETHE1 antibody 27786-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Cell Death Dis

Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma

Authors - Yiwei Liu
View Article