Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14712-1-AP (same clone as 14712-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
WB analysis using 14712-1-AP (same clone as 14712-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
WB analysis of L02 using 14712-1-AP (same clone as 14712-1-PBS)
L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 14712-1-AP (same clone as 14712-1-PBS)
HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
WB analysis of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)
HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
WB analysis of mouse brain using 14712-1-AP (same clone as 14712-1-PBS)
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 14712-1-AP (same clone as 14712-1-PBS)
IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IF Staining of A431 using 14712-1-AP (same clone as 14712-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
IF Staining of PC-3 using 14712-1-AP (same clone as 14712-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
FC experiment of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.
×
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 14712-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

ERp72 Polyclonal antibody, PBS Only

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Cat No. 14712-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

Synonyms

Protein disulfide-isomerase A4, PDIA4, ERp-72, ERp70, ERp 72

Formulation: PBS Only

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

ご購入について

国内販売は「コスモ・バイオ株式会社」を通じて行っております。お見積り・ご注文はお近くの販売代理店へご連絡ください。

国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Overview

Tested Applications
Product Information

データシート［PDF］を印刷

MSDS

Tested Applications

Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

14712-1-PBS targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6431 Product name: Recombinant human ERp72 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-645 aa of BC000425 Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL 相同性解析による交差性が予測される生物種
Full Name	protein disulfide isomerase family A, member 4
Calculated molecular weight	73 kDa
Observed molecular weight	72 kDa
GenBank accession number	BC000425
Gene Symbol	ERp72
Gene ID (NCBI)	9601
RRID	AB_2160973
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13667
Storage Buffer	PBS only{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -80°C.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

WB analysis using 14712-1-AP (same clone as 14712-1-PBS)

WB analysis of L02 using 14712-1-AP (same clone as 14712-1-PBS)

WB analysis of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

WB analysis of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)

WB analysis of mouse brain using 14712-1-AP (same clone as 14712-1-PBS)

IP experiment of HeLa using 14712-1-AP (same clone as 14712-1-PBS)

IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

IHC staining of human liver cancer using 14712-1-AP (same clone as 14712-1-PBS)

IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)

IHC staining of human prostate cancer using 14712-1-AP (same clone as 14712-1-PBS)

IF Staining of A431 using 14712-1-AP (same clone as 14712-1-PBS)

IF Staining of PC-3 using 14712-1-AP (same clone as 14712-1-PBS)

FC experiment of HepG2 using 14712-1-AP (same clone as 14712-1-PBS)