Validation Data Gallery

Filter: AllWBIPIHCIF/ICCFC (Intra)

WB analysis using 14712-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis using 14712-1-AP
Various lysates were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.
WB analysis of L02 using 14712-1-AP
L02 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of mouse brain using 14712-1-AP
mouse brain tissue were subjected to SDS PAGE followed by western blot with 14712-1-AP (ERp72 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis of HepG2 using 14712-1-AP
HepG2 cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 14712-1-AP
HeLa cells were subjected to SDS PAGE followed by western blot with 14712-1-AP (PDIA4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.
IP experiment of HeLa using 14712-1-AP
IP result of anti-ERp72 (IP:14712-1-AP, 4ug; Detection:14712-1-AP 1:500) with HeLa cells lysate 2800ug.
IHC staining of human liver cancer using 14712-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human liver cancer using 14712-1-AP
Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14712-1-AP (ERp72 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human prostate cancer using 14712-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 10x lens).
IHC staining of human prostate cancer using 14712-1-AP
Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 14712-1-AP (ERp72 Antibody) at dilution of 1:50 (under 40x lens).
IF Staining of A431 using 14712-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed A431 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2).
IF Staining of PC-3 using 14712-1-AP
Immunofluorescent analysis of (-20°C Ethanol) fixed PC-3 cells using ERp72 antibody (14712-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).
FC experiment of HepG2 using 14712-1-AP
1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human ERp72 (14712-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

Featured Product
KD/KO Validated

ERp72 Polyclonal antibody

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Cat No. 14712-1-AP

Publications(46)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA

Synonyms

EC:5.3.4.1, ERp 72, ERp70, ERp-72, PDIA4

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

CoraLite® Plus 488

Size/Concentration:

-/ -

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Overview

Tested Applications
Product Information
Publications (46)
Protocols

データシート［PDF］を印刷

MSDS

Tested Applications

Positive WB detected in	mouse brain tissue, HeLa cells, HepG2 cells, L02 cells, mouse liver tissue, rat liver tissue
Positive IP detected in	HeLa cells
Positive IHC detected in	human liver cancer tissue, human prostate cancer tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***
Positive IF/ICC detected in	A431 cells, PC-3 cells
Positive FC (Intra) detected in	HepG2 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:2000-1:10000
Immunoprecipitation (IP)	IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)	IHC : 1:500-1:2000
Immunofluorescence (IF)/ICC	IF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)	FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

KD/KO	See 4 publications below
WB	See 31 publications below
IHC	See 3 publications below
IF	See 16 publications below
IP	See 1 publications below
CoIP	See 1 publications below

Product Information

14712-1-AP targets ERp72 in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human, mouse, pig, monkey
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag6431 Product name: Recombinant human ERp72 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 294-645 aa of BC000425 Sequence: KQVQEFLKDGDDVIIIGVFKGESDPAYQQYQDAANNLREDYKFHHTFSTEIAKFLKVSQGQLVVMQPEKFQSKYEPRSHMMDVQGSTQDSAIKDFVLKYALPLVGHRKVSNDAKRYTRRPLVVVYYSVDFSFDYRAATQFWRSKVLEVAKDFPEYTFAIADEEDYAGEVKDLGLSESGEDVNAAILDESGKKFAMEPEEFDSDTLREFVTAFKKGKLKPVIKSQPVPKNNKGPVKVVVGKTFDSIVMDPKKDVLIEFYAPWCGHCKQLEPVYNSLAKKYKGQKGLVIAKMDATANDVPSDRYKVEGFPTIYFAPSGDKKNPVKFEGGDRDLEHLSKFIEEHATKLSRTKEEL 相同性解析による交差性が予測される生物種
Full Name	protein disulfide isomerase family A, member 4
Calculated molecular weight	73 kDa
Observed molecular weight	72 kDa
GenBank accession number	BC000425
Gene Symbol	ERp72
Gene ID (NCBI)	9601
RRID	AB_2160973
Conjugate	Unconjugated
Form
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P13667
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

PDIA4(Protein disulfide-isomerase A4) is also named as ERP70, ERP72 and belongs to the protein disulfide isomerase family. It catalyzes the rearrangement of -S-S- bonds in proteins. ERp72 is a soluble protein localized in the ER lumen and contains the COOH-terminal retention signal, KEEL. There are 6 cysteine residues in the amino acid sequences of mouse and human ERp72. All of the cysteine residues occur in the internal thioredoxin motif, CGHC(PMID:15475357). The full length protein has a signal peptide with 20 amino acid.

Protocols

Product Specific Protocols
IF protocol for ERp72 antibody 14712-1-AP	Download protocol
IHC protocol for ERp72 antibody 14712-1-AP	Download protocol
IP protocol for ERp72 antibody 14712-1-AP	Download protocol
WB protocol for ERp72 antibody 14712-1-AP	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

Species	Application	Title
mouse	WB	Cell MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation. Authors - Xiaorong Zhang View Article
human	IF	Dev Cell Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation Authors - Wanxin Guo View Article
human	WB	J Thromb Haemost SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia Authors - Jiasheng Zhang View Article
human	WB	Mol Syst Biol Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics Authors - Madison T Wright View Article
		Elife Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation. Authors - Lars Plate View Article
human	WB	Cell Chem Biol Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein. Authors - Lars Plate View Article

Target

Host

Species Reactivity

Species Reactivity

Conjugate

Conjugate

Validation Data Gallery

WB analysis using 14712-1-AP

WB analysis using 14712-1-AP

WB analysis of L02 using 14712-1-AP

WB analysis of mouse brain using 14712-1-AP

WB analysis of HepG2 using 14712-1-AP

WB analysis of HeLa using 14712-1-AP

IP experiment of HeLa using 14712-1-AP

IHC staining of human liver cancer using 14712-1-AP

IHC staining of human liver cancer using 14712-1-AP

IHC staining of human prostate cancer using 14712-1-AP

IHC staining of human prostate cancer using 14712-1-AP

IF Staining of A431 using 14712-1-AP

IF Staining of PC-3 using 14712-1-AP

FC experiment of HepG2 using 14712-1-AP

ERp72 Polyclonal antibody

ERp72 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

-/ -

Tested Applications

Recommended dilution

Published Applications

Product Information

Background Information

Protocols

Publications

MicroRNA Directly Enhances Mitochondrial Translation during Muscle Differentiation.

Mitochondrial CCN1 drives ferroptosis via fatty acid β-oxidation

SINE-VNTR-Alu retrotransposon insertion as a novel mutational event underlying Glanzmann thrombasthenia

Time-resolved interactome profiling deconvolutes secretory protein quality control dynamics

Small molecule proteostasis regulators that reprogram the ER to reduce extracellular protein aggregation.

Quantitative Interactome Proteomics Reveals a Molecular Basis for ATF6-Dependent Regulation of a Destabilized Amyloidogenic Protein.