Validation Data Gallery
Tested Applications
Recommended dilution
| Application | Dilution |
|---|---|
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Product Information
29755-1-PBS targets Eosinophil peroxidase in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag30561 Product name: Recombinant human EPX protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 580-673 aa of NM_000502 Sequence: LSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLLPGARVGPLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSR 相同性解析による交差性が予測される生物種 |
| Full Name | eosinophil peroxidase |
| Calculated molecular weight | 81 kDa |
| Observed molecular weight | 55 kDa |
| GenBank accession number | NM_000502 |
| Gene Symbol | EPX |
| Gene ID (NCBI) | 8288 |
| RRID | AB_3086154 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P11678 |
| Storage Buffer | PBS only{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -80°C. |
Background Information
EPX, also known as eosinophil peroxidase, is a cationic heme‑containing peroxidase enzyme that is predominantly stored in the specific granules of eosinophilic granulocytes. Encoded by the EPX gene located on human chromosome 17q13.1, the enzyme is synthesized as a single‑chain precursor that undergoes proteolytic cleavage to form a heavy and a light chain, which remain covalently associated in the mature protein. Upon eosinophil activation by immunological or inflammatory stimuli, EPX is secreted into the extracellular milieu where it catalyzes the hydrogen peroxide‑dependent oxidation of halides (bromide, chloride) and thiocyanate to generate potent cytotoxic oxidants, such as hypobromous acid and hypothiocyanous acid. These reactive species are essential for the eosinophil's effector function against helminthic parasites and certain bacteria, but they also exert profound pro‑inflammatory and tissue‑damaging effects when released inappropriately. Indeed, elevated EPX levels and activity have been implicated in the pathogenesis of allergic asthma, atopic dermatitis, and other eosinophilic disorders, making the enzyme not only a useful diagnostic biomarker but also an emerging therapeutic target for modulating eosinophil‑driven inflammation.