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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPX Polyclonal antibody (29755-1-AP)

    WB analysis using 29755-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 29755-1-AP (EPX antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human ovary tumor using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human ovary tumor tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human ovary tumor using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human hepatocirrhosis tissue using EPX Polyclonal antibody (29755-1-AP)

    IHC staining of human hepatocirrhosis using 29755-1-AP

    Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 29755-1-AP (EPX antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of mouse lung tissue using EPX Polyclonal antibody (29755-1-AP)

    IF Staining of mouse lung using 29755-1-AP

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse lung tissue using EPX antibody (29755-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

EPX Polyclonal antibody

EPX Polyclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 29755-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, pig

Applications

WB, IHC, IF-P, ELISA

Synonyms

EPX/ PERE, eosinophil peroxidase

Formulation:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

ご購入について

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国内在庫・納期について

約2万点のプロテインテック製品をコスモバイオ社物流センター（国内）に在庫しています。国内在庫の有無はコスモバイオ社ホームページの「品番検索」でカタログ番号を検索して確認できます。

保証・サポートについて

テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Positive WB detected inpig bone marrow tissue
Positive IHC detected inhuman hepatocirrhosis tissue, human ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse lung tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

29755-1-AP targets EPX in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, pig samples.

Tested Reactivity human, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag30561

Product name: Recombinant human EPX protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 580-673 aa of NM_000502

Sequence: LSQPRNLAQLSRVLKNQDLARKFLNLYGTPDNIDIWIGAIAEPLLPGARVGPLLACLFENQFRRARDGDRFWWQKRGVFTKRQRKALSRISLSR

相同性解析による交差性が予測される生物種
Full Name eosinophil peroxidase
Calculated molecular weight 81 kDa
Observed molecular weight55 kDa
GenBank accession numberNM_000502
Gene Symbol EPX
Gene ID (NCBI) 8288
RRIDAB_3086154
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP11678
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
IHC protocol for EPX antibody 29755-1-APDownload protocol
WB protocol for EPX antibody 29755-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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