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Validation Data Gallery

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  • Western Blot (WB) analysis of A431 cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of A431 using 68330-1-Ig (same clone as 68330-1-PBS)

    A431 cells were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68330-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HepG2 cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of HepG2 using 68330-1-Ig (same clone as 68330-1-PBS)

    WB result of EPS8L2 antibody (68330-1-Ig; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-EPS8L2 transfected HepG2 cells. This data was developed using the same antibody clone with 68330-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of rat skin tissue using EPS8L2 Monoclonal antibody (68330-1-Ig)

    WB analysis of rat skin using 68330-1-Ig (same clone as 68330-1-PBS)

    rat skin tissue were subjected to SDS PAGE followed by western blot with 68330-1-Ig (EPS8L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 68330-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using EPS8L2 Monoclonal antibody (68330-1-Ig)

    IF Staining of HeLa using 68330-1-Ig (same clone as 68330-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using EPS8L2 antibody (68330-1-Ig, Clone: 2E3B1 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68330-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EPS8L2 Monoclonal antibody, PBS Only

EPS8L2 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 68330-1-PBS
Clone No.2E3B1

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse, Rat

Applications

WB, IF/ICC, Indirect ELISA

Synonyms

EPS8 like 2, EPS8 like protein 2, EPS8 related protein 2, EPS8L2, EPS8R2, FLJ16738, FLJ21935, FLJ22171

Formulation:  PBS Only
PBS, Azide, Glycerol
PBS Only
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

68330-1-PBS targets EPS8L2 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Reactivity Human, Mouse, Rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen

CatNo: Ag16808

Product name: Recombinant human EPS8L2 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 389-715 aa of BC002474

Sequence: MSLWESLGESWMRPRSEWPREPQVPLYVPKFHSGWEPPVDVLQEAPWEVEGLASAPIEEVSPVSRQSIRNSQKHSPTSEPTPPGDALPPVSSPHTHRGCQPTPAMAKYVKILYDFTARNANELSVLKDEVLEVLEDGRQWWKLRSRSGQAGYVPCNILGEARPEDAGAPFEQAGQKYWGPASPTHKLPPSFPGNKDELMQHMDEVNDELIRKISNIRAQPQRHFRVERSQPVSQPLTYESGPDEVRAWLEAKAFSPRIVENLGILTGPQLFSLNKEELKKVCGEEGVRVYSQLTMQKAFLEKQQSGSELEELMNKFHSMNQRRGEDS

相同性解析による交差性が予測される生物種
Full Name EPS8-like 2
Calculated molecular weight 715 aa, 81 kDa
Observed molecular weight81-85 kDa
GenBank accession numberBC002474
Gene Symbol EPS8L2
Gene ID (NCBI) 64787
Conjugate Unconjugated
Form
FormLiquid
Purification MethodProtein A purification
UNIPROT IDQ9H6S3
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.

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