Validation Data Gallery
Tested Applications
| Positive WB detected in | rat skin tissue, A431 cells, HepG2 cells, A549 cells，LNCaP cells，HeLa cells，HepG2 cells, mouse skin tissue |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
68330-1-Ig targets EPS8L2 in WB, IF/ICC, ELISA applications and shows reactivity with Human, Mouse, Rat samples.
| Tested Reactivity | Human, Mouse, Rat |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16808 Product name: Recombinant human EPS8L2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 389-715 aa of BC002474 Sequence: MSLWESLGESWMRPRSEWPREPQVPLYVPKFHSGWEPPVDVLQEAPWEVEGLASAPIEEVSPVSRQSIRNSQKHSPTSEPTPPGDALPPVSSPHTHRGCQPTPAMAKYVKILYDFTARNANELSVLKDEVLEVLEDGRQWWKLRSRSGQAGYVPCNILGEARPEDAGAPFEQAGQKYWGPASPTHKLPPSFPGNKDELMQHMDEVNDELIRKISNIRAQPQRHFRVERSQPVSQPLTYESGPDEVRAWLEAKAFSPRIVENLGILTGPQLFSLNKEELKKVCGEEGVRVYSQLTMQKAFLEKQQSGSELEELMNKFHSMNQRRGEDS 相同性解析による交差性が予測される生物種 |
| Full Name | EPS8-like 2 |
| Calculated molecular weight | 715 aa, 81 kDa |
| Observed molecular weight | 81-85 kDa |
| GenBank accession number | BC002474 |
| Gene Symbol | EPS8L2 |
| Gene ID (NCBI) | 64787 |
| RRID | AB_2935402 |
| Conjugate | Unconjugated |
| Form | |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | Q9H6S3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol{{ptg:BufferTemp}}7.3 |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
EPS8L2 (epidermal growth factor receptor pathway substrate 8 L2), a member of the Eps8-like protein family, is a newly identified hair bundle protein that is localized at the tips of stereocilia of both cochlear and vestibular hair cells.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for EPS8L2 antibody 68330-1-Ig | Download protocol |
| WB protocol for EPS8L2 antibody 68330-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |