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  • Featured Product
  • KD/KO Validated

CoraLite®594-conjugated EPLIN Monoclonal antibody

EPLIN Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG1

Reactivity

human, rat

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

6E6E6

Cat no : CL594-66071

Synonyms

EPLIN, FLJ38853, LIM domain and actin binding 1, LIMA1, SREBP3

Validation Data Gallery

Applications:
  • IF Staining of HeLa using CL594-66071

    IF Staining of HeLa using CL594-66071

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CL594-66071 (EPLIN antibody) at dilution of 1:100.

Tested Applications

Positive IF detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 66071-1-Ig

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of CL594-66071 is EPLIN Fusion Protein expressed in E. coli.

Tested Reactivity human, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen EPLIN fusion protein Ag13288 相同性解析による交差性が予測される生物種
Full Name LIM domain and actin binding 1
Calculated molecular weight 85 kDa
Observed molecular weight 90 kDa, 110 kDa
GenBank accession numberBC001247
Gene symbol LIMA1
Gene ID (NCBI) 51474
RRIDAB_2883502
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission maxima wavelengths588 nm / 604 nm
Form Liquid
Purification Method Protein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

EPLIN is a cytoskeletal protein that is preferentially expressed in epithelial cells and is implicated in regulation of cytoskeletal dynamics and cytokinesis. In human, there are two known isoforms, EPLIN-a and -b, generated by alternative promoter usage from a single gene. EPLIN-a expression is often down-regulated in cancerous cells and tissues. This antibody recognizes both of isoforms of EPLN.

Protocols

Product Specific Protocols
IF protocol for CL594 EPLIN antibody CL594-66071Download protocol
Standard Protocols
Click here to view our Standard Protocols