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Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using EPHB4 Polyclonal antibody (20883-1-AP)

    WB analysis using 20883-1-AP (same clone as 20883-1-PBS)

    HT-29 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HeLa cells using EPHB4 Polyclonal antibody (20883-1-AP)

    WB analysis of HeLa using 20883-1-AP (same clone as 20883-1-PBS)

    HeLa cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of HT-1080 cells using EPHB4 Polyclonal antibody (20883-1-AP)

    WB analysis of HT-1080 using 20883-1-AP (same clone as 20883-1-PBS)

    HT-1080 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of DU 145 cells using EPHB4 Polyclonal antibody (20883-1-AP)

    WB analysis of DU 145 using 20883-1-AP (same clone as 20883-1-PBS)

    DU 145 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of PC-3 cells using EPHB4 Polyclonal antibody (20883-1-AP)

    WB analysis of PC-3 using 20883-1-AP (same clone as 20883-1-PBS)

    PC-3 cells were subjected to SDS PAGE followed by western blot with 20883-1-AP (EPHB4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 20883-1-AP (EPHB4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human lung cancer tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human lung cancer using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human lung cancer using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human pancreas tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human pancreas using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human pancreas using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunohistochemistry (IHC) staining of human placenta tissue using EPHB4 Polyclonal antibody (20883-1-AP)

    IHC staining of human placenta using 20883-1-AP (same clone as 20883-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human placenta using 20883-1-AP (EPHB4 antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Immunofluorescence (IF) / fluorescent staining of HT-29 cells using EPHB4 Polyclonal antibody (20883-1-AP)

    IF Staining of HT-29 using 20883-1-AP (same clone as 20883-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HT-29 cells using EPHB4 antibody (20883-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 20883-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

EPHB4 Polyclonal antibody, PBS Only

EPHB4 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 20883-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

EC:2.7.10.1, EPH receptor B4, Ephrin type B receptor 4, Ephrin type-B receptor 4, HTK

Formulation:  PBS Only
PBS, Azide, Glycerol
Conjugate:  Unconjugated
Size/Concentration: 

-/ -

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テクニカルサポートまたはご購入後1年間の交換／補填対応を承ります。詳細はこちらをご覧ください。

Tested Applications

Recommended dilution

ApplicationDilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

20883-1-PBS targets EPHB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen

CatNo: Ag10033

Product name: Recombinant human EPHB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 639-987 aa of BC052804

Sequence: KKESCVAIKTLKGGYTERQRREFLSEASIMGQFEHPNIIRLEGVVTNSMPVMILTEFMENGALDSFLRLNDGQFTVIQLVGMLRGIASGMRYLAEMSYVHRDLAARNILVNSNLVCKVSDFGLSRFLEENSSDPTYTSSLGGKIPIRWTAPEAIAFRKFTSASDAWSYGIVMWEVMSFGERPYWDMSNQDVINAIEQDYRLPPPPDCPTSLHQLMLDCWQKDRNARPRFPQVVSALDKMIRNPASLKIVARENGGASHPLLDQRQPHYSAFGSVGEWLRAIKMGRYEESFAAAGFGSFELVSQISAEDLLRIGVTLAGHQKKILASVQHMKSQAKPGTPGGTGGPAPQY

相同性解析による交差性が予測される生物種
Full Name EPH receptor B4
Calculated molecular weight 987 aa, 108 kDa
Observed molecular weight 110-120 kDa
GenBank accession numberBC052804
Gene Symbol EPHB4
Gene ID (NCBI) 2050
RRIDAB_10897158
Conjugate Unconjugated
Form
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDP54760
Storage Buffer PBS only{{ptg:BufferTemp}}7.3
Storage ConditionsStore at -80°C.

Background Information

EPHB4 (Ephrin type-B receptor 4) is a member of the Eph receptor tyrosine kinase family, the largest group of receptor tyrosine kinases. It binds ephrin-B ligands to regulate embryonic vascular development, lymphangiogenesis, and adult angiogenesis. Aberrant EPHB4 signaling promotes tumor progression, metastasis, and therapy resistance in various cancers, including breast, prostate, and colorectal malignancies. As a key regulator of tumor vasculature and the tumor microenvironment, EPHB4 represents an emerging therapeutic target for anti-cancer drug development and a potential prognostic biomarker.

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